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An investigation on...
An investigation on the mechanism of sublimed DHB matrix on molecular ion yields in SIMS imaging of brain tissue
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- Dowlatshahi Pour, Masoumeh, 1976 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Malmberg, Per, 1974 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ewing, Andrew G, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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(creator_code:org_t)
- 2016-02-27
- 2016
- English.
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In: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 408:12, s. 3071-3081
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Abstract
Subject headings
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- We have characterized the use of sublimation to deposit matrix-assisted laser desorption/ionization (MALDI) matrices in secondary ion mass spectrometry (SIMS) analysis, i.e. matrix-enhanced SIMS (ME-SIMS), a common surface modification method to enhance sensitivity for larger molecules and to increase the production of intact molecular ions. We use sublimation to apply a thin layer of a conventional MALDI matrix, 2,5-dihydroxybenzoic acid (DHB), onto rat brain cerebellum tissue to show how this technique can be used to enhance molecular yields in SIMS while still retaining a lateral resolution around 2 mu m and also to investigate the mechanism of this enhancement. The results here illustrate that cholesterol, which is a dominant lipid species in the brain, is decreased on the tissue surface after deposition of matrix, particularly in white matter. The decrease of cholesterol is followed by an increased ion yield of several other lipid species. Depth profiling of the sublimed rat brain reveals that the lipid species are de facto extracted by the DHB matrix and concentrated in the top most layers of the sublimed matrix. This extraction/concentration of lipids directly leads to an increase of higher mass lipid ion yield. It is also possible that the decrease of cholesterol decreases the potential suppression of ion yield caused by cholesterol migration to the tissue surface. This result provides us with significant insights into the possible mechanisms involved when using sublimation to deposit this matrix in ME-SIMS.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- ME-SIMS mechanism
- Cholesterol migration
- MS imaging
- desorption/ionization mass-spectrometry
- tof-sims
- sample preparation
- maldi-ms
- enhancement
- sensitivity
- discovery
- surface
- lipids
- beams
- Biochemistry & Molecular Biology
- Chemistry
- sample preparation
Publication and Content Type
- ref (subject category)
- art (subject category)
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