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Increased Expressio...
Increased Expression of the Diabetes Gene SOX4 Reduces Insulin Secretion by Impaired Fusion Pore Expansion
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- Collins, S. C. (author)
- University of Oxford
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- Do, H. W. (author)
- University of Oxford
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- Hastoy, B. (author)
- University of Oxford
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- Rosengren, Anders H. (author)
- Lund University,Lunds universitet,Diabetes,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Adam, J. (author)
- University of Oxford
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- Chibalina, M. V. (author)
- University of Oxford
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- Galvanovskis, Juris (author)
- University of Oxford
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- Godazgar, M. (author)
- University of Oxford
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- Lee, S. (author)
- University of Oxford
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- Goldsworthy, M. (author)
- Mary Lyon Centre Pathology
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- Salehi, Albert (author)
- Lund University,Lunds universitet,Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology,Diabetes,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Tarasov, A. I. (author)
- University of Oxford
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Rosengren, A. H. (author)
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- Cox, R. (author)
- Mary Lyon Centre Pathology
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- Rorsman, Patrik (author)
- University of Gothenburg,University of Oxford
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(creator_code:org_t)
- 2016-03-18
- 2016
- English.
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In: Diabetes. - : American Diabetes Association. - 0012-1797 .- 1939-327X. ; 65:7, s. 1952-1961
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Abstract
Subject headings
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- The transcription factor Sox4 has been proposed to underlie the increased type 2 diabetes risk linked to an intronic single nucleotide polymorphism in CDKAL1. In a mouse model expressing a mutant form of Sox4, glucose-induced insulin secretion is reduced by 40% despite normal intracellular Ca2+ signaling and depolarization-evoked exocytosis. This paradox is explained by a fourfold increase in kiss-and-run exocytosis (as determined by single-granule exocytosis measurements) in which the fusion pore connecting the granule lumen to the exterior expands to a diameter of only 2 nm, which does not allow the exit of insulin. Microarray analysis indicated that this correlated with an increased expression of the exocytosis-regulating protein Stxbp6. In a large collection of human islet preparations (n = 63), STXBP6 expression and glucose induced insulin secretion correlated positively and negatively with SOX4 expression, respectively. Overexpression of SOX4 in the human insulin-secreting cell EndoC-beta H2 interfered with granule emptying and inhibited hormone release, the latter effect reversed by silencing STXBP6. These data suggest that increased SOX4 expression inhibits insulin secretion and increased diabetes risk by the upregulation of STXBP6 and an increase in kiss- and-run exocytosis at the expense of full fusion. We propose that pharmacological interventions promoting fusion pore expansion may be effective in diabetes therapy.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- pancreatic beta-cells
- kiss-and-run
- genome-wide association
- vesicle
- exocytosis
- risk
- release
- endocytosis
- variants
- obesity
- islets
- Endocrinology & Metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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Diabetes
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- By the author/editor
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Collins, S. C.
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Do, H. W.
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Hastoy, B.
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Rosengren, Ander ...
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Adam, J.
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Chibalina, M. V.
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show more...
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Galvanovskis, Ju ...
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Godazgar, M.
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Lee, S.
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Goldsworthy, M.
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Salehi, Albert
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Tarasov, A. I.
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Rosengren, A. H.
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Cox, R.
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Rorsman, Patrik
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show less...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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Diabetes
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University of Gothenburg
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Lund University