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- Nogly, P. (author)
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
- Article/chapterEnglish2016
Publisher, publication year, extent ...
- 2016-08-22
- Springer Science and Business Media LLC,2016
Numbers
- LIBRIS-ID:oai:gup.ub.gu.se/242001
- https://gup.ub.gu.se/publication/242001URI
- https://doi.org/10.1038/ncomms12314DOI
- https://research.chalmers.se/publication/242001URI
Supplementary language notes
- Language:English
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- SwePubSwePub
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Notes
- Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 angstrom resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
Subject headings and genre
- NATURVETENSKAP Biologi hsv//swe
- NATURAL SCIENCES Biological Sciences hsv//eng
- NATURVETENSKAP Kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences hsv//eng
- x-ray-diffraction
- protein-coupled receptors
- coherent-light source
- femtosecond crystallography
- membrane-proteins
- radiation-damage
- macromolecular crystallography
- structural alterations
- photocycle
- kinetics
- bacteriorhodopsin
- Science & Technology - Other Topics
- femtosecond crystallography
Added entries (persons, corporate bodies, meetings, titles ...)
- Panneels, V. (author)
- Nelson, G. (author)
- Gati, C. (author)
- Kimura, T. (author)
- Milne, C. (author)
- Milathianaki, D. (author)
- Kubo, M. (author)
- Wu, W. T. (author)
- Conrad, C. (author)
- Coe, J. (author)
- Bean, R. (author)
- Zhao, Y. (author)
- Båth, Petra,1988Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:gu)xbatpe (author)
- Dods, Robert,1989Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:gu)xdodsr (author)
- Harimoorthy, RajivGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:gu)xharra (author)
- Beyerlein, K. R. (author)
- Rheinberger, J. (author)
- James, D. (author)
- DePonte, D. (author)
- Li, C. F. (author)
- Sala, L. (author)
- Williams, G. J. (author)
- Hunter, M. S. (author)
- Koglin, J. E. (author)
- Berntsen, Peter,1974Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:cth)peterber (author)
- Nango, E. (author)
- Iwata, S. (author)
- Chapman, H. N. (author)
- Fromme, P. (author)
- Frank, M. (author)
- Abela, R. (author)
- Boutet, S. (author)
- Barty, A. (author)
- White, T. A. (author)
- Weierstall, U. (author)
- Spence, J. (author)
- Neutze, Richard,1969Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:cth)xneuri (author)
- Schertler, G. (author)
- Standfuss, J. (author)
- Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)
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- In:Nature Communications: Springer Science and Business Media LLC72041-1723
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