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  • Edlund, PetraGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (author)

The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography

  • Article/chapterEnglish2016

Publisher, publication year, extent ...

  • 2016-10-19
  • Springer Science and Business Media LLC,2016

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/245514
  • https://gup.ub.gu.se/publication/245514URI
  • https://doi.org/10.1038/srep35279DOI

Supplementary language notes

  • Language:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 angstrom resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 angstrom resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.

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  • Takala, HeikkiGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (author)
  • Claesson, Elin,1989Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xclael (author)
  • Henry, LéocadieGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xheleo (author)
  • Dods, Robert,1989Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xdodsr (author)
  • Lehtivuori, Heli (author)
  • Panman, Matthijs R,1983Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xpanmb (author)
  • Pande, K. (author)
  • White, T. (author)
  • Nakane, T. (author)
  • Berntsson, Oskar,1989Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xboska (author)
  • Gustavsson, Emil,1987Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xguemi (author)
  • Båth, Petra,1988Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xbatpe (author)
  • Modi, V. (author)
  • Roy-Chowdhury, S. (author)
  • Zook, J. (author)
  • Berntsen, P. (author)
  • Pandey, S. (author)
  • Poudyal, I. (author)
  • Tenboer, J. (author)
  • Kupitz, C. (author)
  • Barty, A. (author)
  • Fromme, P. (author)
  • Koralek, J. D. (author)
  • Tanaka, T. (author)
  • Spence, J. (author)
  • Liang, M. L. (author)
  • Hunter, M. S. (author)
  • Boutet, S. (author)
  • Nango, E. (author)
  • Moffat, K. (author)
  • Groenhof, G. (author)
  • Ihalainen, J. (author)
  • Stojkovic, E. A. (author)
  • Schmidt, M. (author)
  • Westenhoff, Sebastian,1978Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xwests (author)
  • Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

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  • In:Scientific Reports: Springer Science and Business Media LLC62045-2322

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