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Heme Oxygenase-1 Me...
Heme Oxygenase-1 Mediates Neuroprotection Conferred by Argon in Combination with Hypothermia in Neonatal Hypoxia-Ischemia Brain Injury
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Zhao, H. L. (author)
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Mitchell, S. (author)
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Koumpa, S. (author)
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Cui, Y. T. (author)
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Lian, Q. Q. (author)
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- Hagberg, Henrik, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology
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Johnson, M. R. (author)
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Takata, M. (author)
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Ma, D. Q. (author)
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(creator_code:org_t)
- Ovid Technologies (Wolters Kluwer Health), 2016
- 2016
- English.
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In: Anesthesiology. - : Ovid Technologies (Wolters Kluwer Health). - 0003-3022. ; 125:1, s. 180-192
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http://spiral.imperi...
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https://doi.org/10.1...
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Abstract
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- Background: Hypoxic-ischemic encephalopathy is a major cause of mortality and disability in the newborn. The authors investigated the protective effects of argon combined with hypothermia on neonatal rat hypoxic-ischemic brain injury. Methods: In in vitro studies, rat cortical neuronal cell cultures were challenged by oxygen and glucose deprivation for 90 min and exposed to 70% Ar or N-2 with 5% CO2 balanced with O-2, at 33 degrees C for 2 h. Neuronal phospho-Akt, heme oxygenase-1 and phospho-glycogen synthase kinase-3a expression, and cell death were assessed. In in vivo studies, neonatal rats were subjected to unilateral common carotid artery ligation followed by hypoxia (8% O-2 balanced with N-2 and CO2) for 90 min. They were exposed to 70% Ar or N-2 balanced with oxygen at 33 degrees, 35 degrees, and 37 degrees C for 2 h. Brain injury was assessed at 24 h or 4 weeks after treatment. Results: In in vitro studies, argon-hypothermia treatment increased phospho-Akt and heme oxygenase-1 expression and significantly reduced the phospho-glycogen synthase kinase-3 beta Tyr-216 expression, cytochrome C release, and cell death in oxygen-glucose deprivation-exposed cortical neurons. In in vivo studies, argon-hypothermia treatment decreased hypoxia/ischemia-induced brain infarct size (n = 10) and both caspase-3 and nuclear factor-kappa B activation in the cortex and -hippocampus. It also reduced hippocampal astrocyte activation and proliferation. Inhibition of phosphoinositide-3-kinase (PI 3K)/Akt pathway through LY294002 attenuated cerebral protection conferred by argon-hypothermia treatment (n = 8). Conclusion: Argon combined with hypothermia provides neuroprotection against cerebral hypoxia-ischemia damage in neonatal rats, which could serve as a new therapeutic strategy against hypoxic-ischemic encephalopathy.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Anestesi och intensivvård (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Anesthesiology and Intensive Care (hsv//eng)
Keyword
- cell-death
- term neuroprotection
- neuronal injury
- noble-gases
- xenon
- rats
- asphyxia
- model
- akt
- neurodegeneration
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Zhao, H. L.
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Mitchell, S.
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Koumpa, S.
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Cui, Y. T.
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Lian, Q. Q.
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Hagberg, Henrik, ...
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show more...
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Johnson, M. R.
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Takata, M.
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Ma, D. Q.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Anesthesiology a ...
- Articles in the publication
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Anesthesiology
- By the university
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University of Gothenburg