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High-throughput ana...
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Adamczyk, Barbara,1985Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
(author)
High-throughput analysis of the plasma N-glycome by UHPLC
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2016-10-15
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New York :Springer,2017
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LIBRIS-ID:oai:gup.ub.gu.se/247970
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https://gup.ub.gu.se/publication/247970URI
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https://doi.org/10.1007/978-1-4939-6493-2_8DOI
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Subject category:vet swepub-contenttype
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Subject category:kap swepub-publicationtype
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The understanding of glycosylation alterations in health and disease has evolved significantly and glycans are considered to be relevant biomarker candidates. High-throughput analytical technologies capable of generating high-quality, large-scale glycoprofiling data are in high demand. Here, we describe an automated sample preparation workflow and analysis of N-linked glycans from plasma samples using hydrophilic interaction liquid chromatography with fluorescence detection on an ultrahigh-performance liquid chromatography (UHPLC) instrument. Samples are prepared in 96-well plates and the workflow features rapid glycoprotein denaturation, enzymatic glycan release, glycan purification on solid-supported hydrazide, fluorescent labeling, and post-labeling cleanup with solid-phase extraction. The development of a novel approach for plasma N-glycan analysis and its implementation on a robotic platform significantly reduces the time required for sample preparation and minimizes technical variation. It is anticipated that the developed method will contribute to expanding high-throughput capabilities to analyze protein glycosylation. © Springer Science+Business Media New York 2017.
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Stöckmann, H.
(author)
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O’Flaherty, R.
(author)
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Karlsson, Niclas G.,1966Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xkanic
(author)
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Rudd, P. M.
(author)
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
(creator_code:org_t)
Related titles
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In:High-Throughput Glycomics and Glycoproteomics, Eds: Gordan Lauc & Manfred WuhrerNew York : Springer, s. 97-1081064-37459781493964918
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