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Microcystin-LR caus...
Microcystin-LR causes sexual hormone disturbance in male rat by targeting gonadotropin-releasing hormone neurons
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Wang, X. T. (author)
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Ding, J. (author)
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- Xiang, Zou (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Jiang, P. P. (author)
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Du, J. (author)
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Han, X. D. (author)
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(creator_code:org_t)
- Elsevier BV, 2016
- 2016
- English.
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In: Toxicon. - : Elsevier BV. - 0041-0101. ; 123, s. 45-55
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Abstract
Subject headings
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- Microcystin-LR (MC-LR), which generates strong reproductive toxicity, reduces serum testosterone level without directly damaging Leydig cells. The purpose of this study was to identify the target cells of MC-LR in the rat hypothalamic-pituitary axis and to investigate the underlying mechanisms. We found that the gonadotropin-releasing hormone (GnRH) neurons were the direct target of MC-LR. In vivo results showed that upon exposure to rats, cells around the third ventricle of hypothalamus underwent apoptosis. Gnrh1 expression was steadily decreased. The serum levels of GnRH, luteinizing hormone, follicle-stimulating hormone and testosterone showed a similar pattern of early-stage increase and late stage decline. After treatment with MC-LR in immortalized hypothalamic (GTI-7) neurons, increase in cellular Ca2+ and ATP resulted in downregulation of the transcriptional activators Oct-1, Otx-2, Pbxl a and Dlx2, and upregulation of the transcriptional repressor c-Jun, which may mechanistically account for the downregulation of GnRH synthesis. Ca2+ and ATP could also stimulate the release of GnRH, contributing to its unique serum release pattern. (C) 2016 Published by Elsevier Ltd.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Microcystin-LR
- GnRH neuron
- GT1-7 cells
- Hormones
- gene-expression
- cyanobacterial toxins
- induced apoptosis
- embryo-larval
- in-vitro
- cells
- exposure
- transcription
- glutamate
- zebrafish
- Pharmacology & Pharmacy
- Toxicology
- hwanzelfukuda m
- 1989
- nature
- v338
- p161
Publication and Content Type
- ref (subject category)
- art (subject category)
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