Proteotyping for Rapid Identifications of Clinically-Relevant Infectious Bacteria

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Salvà-Serra, Francisco,1989Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine (author)

Proteotyping for Rapid Identifications of Clinically-Relevant Infectious Bacteria

Article/chapterEnglish2016

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2016

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LIBRIS-ID:oai:gup.ub.gu.se/249207
https://gup.ub.gu.se/publication/249207URI

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Language:English

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SwePubSwePub

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Subject category:vet swepub-contenttype
Subject category:kon swepub-publicationtype

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Aims The development and application of novel identifications and diagnostics of pathogenic bacteria, virulence and antibiotic resistance factors, to enhance treatment of infectious diseases and to address the pandemic of antimicrobial resistance (1). To apply mass spectrometry (MS)-based ‘proteotyping’, a rapid proteomic-genomic method to identify and use cell biomarkers for pathogen identification and detection of targeted metabolic functions (www.tailored-treatment.eu/). Methods and results The proteins of intact bacterial cells or cell-fractions are bound to a membrane surface, using the patented (WO2006068619) Lipid-based Protein Immobilization (LPI) technology. Peptides are generated from bound proteins, using enzymatic digestion, separated and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The MS profiles are compared to those of reference peptide sequences and the peptide sequences are compared against a curated in-house database of genome sequences. Conclusions Analyses of bacterial cell peptides identified protein biomarkers of infectious bacteria, at the species-level, virulence and antibiotic resistance factors. Model samples have been ‘proteotyped’, using well-characterized reference strains, and an enhanced whole-genome sequence database, to demonstrate ‘proof-of-concept’, and the method been applied directly to the analyses of clinical samples, without prior cultivation. Significance of study Proteotyping demonstrates the potential for proteomics-based analyses for detecting expressed genomic markers of bacterial species, virulence and antibiotic resistance, for the identifications and diagnostics of infectious microorganisms. References Cohen A, Bont L, Engelhard D, Moore E, Fernández D, Kreisberg-Greenblatt R, Oved K, Eden E, Hayes J (2015). A multifaceted 'omics' approach for addressing the challenge of antimicrobial resistance. Future Microbiol. 10:365-376

Subject headings and genre

NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Biologisk systematik hsv//swe
NATURAL SCIENCES Biological Sciences Biological Systematics hsv//eng

Added entries (persons, corporate bodies, meetings, titles ...)

Jakobsson, Hedvig EGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xenhed (author)
Gonzales-Siles, LuciaGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xgonlu (author)
Karlsson, Roger,1975Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xkarro (author)
Boulund, Fredrik,1985Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper, matematisk statistik,Department of Mathematical Sciences, Mathematical Statistics(Swepub:gu)xboufr (author)
Jaén-Luchoro, DanielGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xjaeda (author)
Bennasar-Figueras, A (author)
Kristiansson, Erik,1978Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper, matematisk statistik,Department of Mathematical Sciences, Mathematical Statistics(Swepub:gu)xkrier (author)
Moore, Edward R.B.1954Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xmooed (author)
Göteborgs universitetInstitutionen för biomedicin, avdelningen för infektionssjukdomar (creator_code:org_t)

Related titles

In:Programme of the XXXV ECCO Meeting 2016

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

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