Fibronectin stimulates transcription of the platelet-derived growth factor beta-receptor in cultured rat aortic smooth muscle cells.

Fibronectin seems to play an important role in promoting the characteristic changes of vascular smooth muscle cells in diabetes mellitus including overexpression of the platelet-derived growth factor beta-receptor. To determine the regulatory mechanism of the beta-receptor by fibronectin, we have analyzed the effect of fibronectin on the expression of the beta-receptor in cultured rat aortic smooth muscle cells using the beta-receptor promoter/luciferase expression vector system. Fibronectin was found to stimulate the expression of the beta-receptor at the transcriptional level. Both a MEK1 inhibitor PD98059 and a tyrosine kinase inhibitor herbimycin A significantly inhibited the fibronectin-stimulated receptor transcription. Herbimycin A also completely inhibited the fibronectin-stimulated increase in tyrosine phosphorylation of focal adhesion kinase. These data suggest the involvement of the integrin-mediated mitogen-activated protein kinase pathway downstream of fibronectin stimulation in the activation process of the beta-receptor promoter.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Animals
Aorta
cytology
drug effects
Benzoquinones
Calcium-Calmodulin-Dependent Protein Kinases
metabolism
Dose-Response Relationship
Drug
Fibronectins
pharmacology
Flavonoids
pharmacology
Genes
Reporter
Integrins
metabolism
Lactams
Macrocyclic
Luciferases
biosynthesis
genetics
Muscle
Smooth
Vascular
drug effects
Promoter Regions
Genetic
Protein Kinase Inhibitors
Quinones
pharmacology
Rats
Receptor
Platelet-Derived Growth Factor beta
Receptors
Platelet-Derived Growth Factor
biosynthesis
genetics
Recombinant Fusion Proteins
biosynthesis
Rifabutin
analogs & derivatives
Signal Transduction
Transcription
Genetic

Yokote, K (author)
Takemoto, M (author)
Matsumoto, T (author)
Ishisaki, A (author)
Funa, Keiko,1949Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology(Swepub:gu)xfunke (author)
Saito, Y (author)
Mori, S (author)
Göteborgs universitetInstitutionen för anatomi och cellbiologi (creator_code:org_t)

In:Biochemical and biophysical research communications: Elsevier BV251:3, s. 677-800006-291X

By the author/editor: Tamura, K; Yokote, K; Takemoto, M; Matsumoto, T; Ishisaki, A; Funa, Keiko, 194 ...; show more...; Saito, Y; Mori, S; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.