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Nuclear factor Y co...
Nuclear factor Y controls the basal transcription activity of the mouse platelet-derived-growth-factor beta-receptor gene.
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Ishisaki, A (författare)
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Murayama, T (författare)
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Ballagi, A E (författare)
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visa fler...
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- Funa, Keiko, 1949 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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visa färre...
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(creator_code:org_t)
- 1997
- 1997
- Engelska.
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Ingår i: European journal of biochemistry. - 0014-2956. ; 246:1, s. 142-6
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- To determine the regulatory mechanism of the expression of the mouse platelet-derived growth factor (PDGF) beta-receptor gene, a 1.9-kb 5' flanking genomic fragment was cloned and analyzed. Site-directed mutagenesis of a CCAAT motif, located 60 bp upstream of the transcriptional-start site, completely abolished the promoter activity [Ballagi, A. E., Ishisaki, A., Nelin, J.-O. & Funa, K. (1995) Biochem. Biophys. Res. Commun. 210, 165-1751. The sequence around the intact CCAAT motif was protected by in vitro DNase-I-footprinting analysis. Electrophoresis-mobility-shift assays with anti-[nuclear factor Y(NF-Y)]Ig revealed binding of the NF-Y complex to the CCAAT box. Furthermore, the double-stranded oligonucleotides corresponding to the sequence around the CCAAT motif were conjugated with DNA-affinity magnetic beads. The binding proteins were affinity purified and identified as the NF-Y transcription factor by western blotting. Our results indicate that NF-Y controls the basal transcription activity of the mouse PDGF beta-receptor gene.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- 3T3 Cells
- Animals
- Blotting
- Western
- CCAAT-Enhancer-Binding Proteins
- Cloning
- Molecular
- DNA Footprinting
- DNA-Binding Proteins
- isolation & purification
- metabolism
- Electrophoresis
- Polyacrylamide Gel
- Gene Expression Regulation
- Mice
- Mutagenesis
- Site-Directed
- Promoter Regions
- Genetic
- Receptor
- Platelet-Derived Growth Factor beta
- Receptors
- Platelet-Derived Growth Factor
- genetics
- Transcription Factors
- metabolism
- Transfection
- genetics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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