Distinct prostate cancer-related mRNA cargo in extracellular vesicle subsets from prostate cell lines

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: onr:"swepub:oai:gup.ub.gu.se/251133" > Distinct prostate c...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Lázaro-Ibáñez, ElisaGothenburg University,Göteborgs universitet,Krefting Research Centre,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition (author)

Distinct prostate cancer-related mRNA cargo in extracellular vesicle subsets from prostate cell lines

Article/chapterEnglish2017

Publisher, publication year, extent ...

2017-02-01
Springer Science and Business Media LLC,2017

Numbers

LIBRIS-ID:oai:gup.ub.gu.se/251133
https://gup.ub.gu.se/publication/251133URI
https://doi.org/10.1186/s12885-017-3087-xDOI

Supplementary language notes

Language:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Background: Multiple types of extracellular vesicles (EVs), including microvesicles (MVs) and exosomes (EXOs), are released by all cells constituting part of the cellular EV secretome. The bioactive cargo of EVs can be shuffled between cells and consists of lipids, metabolites, proteins, and nucleic acids, including multiple RNA species from non-coding RNAs to messenger RNAs (mRNAs). In this study, we hypothesized that the mRNA cargo of EVs could differ based on the EV cellular origin and subpopulation analyzed. Methods: We isolated MVs and EXOs from PC-3 and LNCaP prostate cancer cells by differential centrifugation and compared them to EVs derived from the benign PNT2 prostate cells. The relative mRNA levels of 84 prostate cancer-related genes were investigated and validated using quantitative reverse transcription PCR arrays. Results: Based on the mRNA abundance, MVs rather than EXOs were enriched in the analyzed transcripts, providing a snapshot of the tumor transcriptome. LNCaP MVs specifically contained significantly increased mRNA levels of NK3 Homeobox 1 (NKX3-1), transmembrane protease serine 2 (TMPRSS2), and tumor protein 53 (TP53) genes, whereas PC-3 MVs carried increased mRNA levels of several genes including, caveolin-2 (CAV2), glutathione S-transferase pi 1 (GSTP1), pescadillo ribosomal biogenesis factor 1 (PES1), calmodulin regulated spectrin associated protein 1 (CAMSAP1), zinc-finger protein 185 (ZNF185), and others compared to PNT2 MVs. Additionally, ETS variant 1 (ETV1) and fatty acid synthase (FASN) mRNAs identified in LNCaP-and PC-3-derived MVs highly correlated with prostate cancer progression. Conclusions: Our study provides new understandings of the variability of the mRNA cargo of MVs and EXOs from different cell lines despite same cancer origin, which is essential to better understand the the proportion of the cell transcriptome that can be detected within EVs and to evaluate their role in disease diagnosis.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
mRNA
Extracellular vesicles
Microvesicles
Exosomes
Prostate cancer
gene-expression profiles
breast-cancer
biological-properties
serine-protease
noncoding rnas
exosomes
microvesicles
progression
hypermethylation
micrornas
Oncology

Added entries (persons, corporate bodies, meetings, titles ...)

Lunavat, Taral RGothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Krefting Research Centre,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition (author)
Jang, Su Chul,1984Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Krefting Research Centre,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition(Swepub:gu)xjangs (author)
Escobedo-Lucea, C. (author)
Oliver-De La Cruz, J. (author)
Siljander, P. (author)
Lötvall, Jan,1956Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Krefting Research Centre,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition(Swepub:gu)xlotja (author)
Yliperttula, M. (author)
Göteborgs universitetKrefting Research Centre (creator_code:org_t)

Related titles

In:Bmc Cancer: Springer Science and Business Media LLC17:921471-2407

Internet link

Find in a library

Bmc Cancer (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Lázaro-Ibáñez, E ...; Lunavat, Taral R; Jang, Su Chul, 1 ...; Escobedo-Lucea, ...; Oliver-De La Cru ...; Siljander, P.; show more...; Lötvall, Jan, 19 ...; Yliperttula, M.; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

Articles in the publication: Bmc Cancer

By the university: University of Gothenburg

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se