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Catch and Release: ...
Catch and Release: rare cell analysis from a functionalised medical wire
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Chen, S. K. (author)
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El-Heliebi, A. (author)
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Tauber, G. (author)
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Langsenlehner, T. (author)
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Potscher, M. (author)
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Kashofer, K. (author)
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Czyz, Z. T. (author)
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Polzer, B. (author)
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Riethdorf, S. (author)
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Kuske, A. (author)
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Leitinger, G. (author)
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Pantel, K. (author)
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- Kroneis, Thomas (author)
- Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center
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Sedlmayr, P. (author)
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(creator_code:org_t)
- 2017-02-24
- 2017
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7
- Related links:
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https://doi.org/10.1...
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Abstract
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- Enumeration and especially molecular characterization of circulating tumour cells (CTCs) holds great promise for cancer management. We tested a modified type of an in vivo enrichment device (Catch& Release) for its ability to bind and detach cancer cells for the purpose of single-cell molecular downstream analysis in vitro. The evaluation showed that single-cell analysis using array comparative genome hybridization (array-CGH) and next generation sequencing (NGS) is feasible. We found array-CGH to be less noisy when whole genome amplification (WGA) was performed with Ampli1 as compared to GenomePlex (DLRS values 0.65 vs. 1.39). Moreover, Ampli1-processed cells allowed detection of smaller aberrations (median 14.0 vs. 49.9 Mb). Single-cell NGS data obtained from Ampli1-processed samples showed the expected non-synonymous mutations (deletion/SNP) according to bulk DNA. We conclude that clinical application of this refined in vivo enrichment device allows CTC enumeration and characterization, thus, representing a promising tool for personalized medicine.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- circulating tumor-cells
- metastatic breast-cancer
- resistant
- prostate-cancer
- multiplex pcr
- genome
- morphology
- survival
- markers
- Science & Technology - Other Topics
- C 08-11
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Chen, S. K.
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El-Heliebi, A.
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Tauber, G.
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Langsenlehner, T ...
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Potscher, M.
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Kashofer, K.
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show more...
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Czyz, Z. T.
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Polzer, B.
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Riethdorf, S.
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Kuske, A.
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Leitinger, G.
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Pantel, K.
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Kroneis, Thomas
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Sedlmayr, P.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Scientific Repor ...
- By the university
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University of Gothenburg