Effect of Spinal Manometers on Cerebrospinal Fluid Amyloid-beta Concentration

• The effect of spinal manometers on cerebrospinal fluid (CSF) amyloid-beta (A beta) concentration was investigated. Pooled human CSF samples were divided in two, one half passed through a manometer into a collection tube, the other transferred directly to a collection tube. CSF was analyzed for A beta 38/40/42 using an electrochemiluminescence immunoassay. Relative to control, use of a manometer decreased A beta 38/40/42 concentration by 5.6% (+/- 1.5SE), 4.4% (+/- 1.7SE), and 4.3% (+/- 2.4SE), respectively. The ratios of A beta(42 : 40), A beta(42 : 38), and A beta(40 : 38) were not affected by manometer treatment. Factors which artificially lower CSF A beta concentrations are relevant to clinical diagnosis for AD and study design.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Neurologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Neurology (hsv//eng)

Keyword

Alzheimer's disease

amyloid-beta

biomarkers

cerebrospinal fluid

manometer

pre-analytical factor

alzheimers association workgroups

preanalytical confounding factors

randomized clinical-trial

quality-control program

diagnostic

guidelines

national institute

tau-protein

disease

biomarkers

recommendations

Neurosciences & Neurology

Publication and Content Type

ref (subject category)

art (subject category)