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- Pichaud, Nicolas,1978Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences (author)
Dynamic changes in cardiac mitochondrial metabolism during warm acclimation in rainbow trout
- Article/chapterEnglish2017
Publisher, publication year, extent ...
- 2017-01-01
- The Company of Biologists,2017
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- LIBRIS-ID:oai:gup.ub.gu.se/254384
- https://gup.ub.gu.se/publication/254384URI
- https://doi.org/10.1242/jeb.152421DOI
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- Language:English
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- Although the mitochondrial metabolism responses to warm acclimation have been widely studied in fish, the time course of this process is less understood. Here, we characterized the changes of rainbow trout (Oncorhynchus mykiss) cardiac mitochondrial metabolism during acute warming from 10 to 16 degrees C, and during the subsequent warm acclimation for 39 days. We repeatedly measured mitochondrial oxygen consumption in cardiac permeabilized fibers and the functional integrity of mitochondria (i.e. mitochondrial coupling and cytochrome c effect) at two assay temperatures (10 and 16 degrees C), as well as the activities of citrate synthase (CS) and lactate dehydrogenase (LDH) at room temperature. LDH and CS activities significantly increased between day 0 (10 degrees C acclimated fish) and day 1 (acute warming to 16 degrees C) while mitochondrial oxygen consumption measured at respective in vivo temperatures did not change. Enzymatic activities and mitochondrial oxygen consumption rates significantly decreased by day 2, and remained stable during warm acclimation (days 2-39). The decrease in rates of oxygen between day 0 and day 1 coincided with an increased cytochrome c effect and a decreased mitochondrial coupling, suggesting a structural/functional impairment of mitochondria during acute warming. We suggest that after 2 days of warm acclimation, a new homeostasis is reached, which may involve the removal of dysfunctional mitochondria. Interestingly, from day 2 onwards, there was a lack of differences in mitochondrial oxygen consumption rates between the assay temperatures, suggesting that warm acclimation reduces the acute thermal sensitivity of mitochondria. This study provides significant knowledge on the thermal sensitivity of cardiac mitochondria that is essential to delineate the contribution of cellular processes to warm acclimation.
Subject headings and genre
- NATURVETENSKAP Biologi hsv//swe
- NATURAL SCIENCES Biological Sciences hsv//eng
- Warm acclimation
- Electron transport system
- Fish
- Heart
- Mitochondria
- Temperature
- cytochrome-c-oxidase
- permeabilized muscle-fibers
- fish
- gillichthys-mirabilis
- unfolded-protein response
- littorina-littorea l
- thermal-acclimation
- temperature-acclimation
- oncorhynchus-mykiss
- time-course
- red muscle
- Life Sciences & Biomedicine - Other Topics
Added entries (persons, corporate bodies, meetings, titles ...)
- Ekström, Andreas,1979Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences(Swepub:gu)xekstr (author)
- Hellgren, KimGothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences (author)
- Sandblom, Erik,1978Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap,Department of Biological and Environmental Sciences(Swepub:gu)xsaner (author)
- Göteborgs universitetInstitutionen för biologi och miljövetenskap (creator_code:org_t)
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- In:Journal of Experimental Biology: The Company of Biologists220:9, s. 1674-16830022-09491477-9145
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