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Lysozyme's lectin-l...
Lysozyme's lectin-like characteristics facilitates its immune defense function
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Zhang, R. Y. (author)
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Wu, L. S. (author)
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Eckert, T. (author)
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Burg-Roderfeld, M. (author)
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Rojas-Macias, M. A. (author)
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Lutteke, T. (author)
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Krylov, V. B. (author)
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Argunov, D. A. (author)
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Datta, A. (author)
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Markart, P. (author)
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Guenther, A. (author)
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Norden, B. (author)
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Schauer, R. (author)
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Bhunia, A. (author)
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Enani, M. A. (author)
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Billeter, M. (author)
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Scheidig, A. J. (author)
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- Nifantiev, Nikolay (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Siebert, H. C. (author)
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(creator_code:org_t)
- 2017-05-30
- 2017
- English.
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In: Quarterly Reviews of Biophysics. - : Cambridge University Press (CUP). - 0033-5835 .- 1469-8994. ; 50, s. 1-12
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Abstract
Subject headings
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- Interactions between human lysozyme (HL) and the lipopolysaccharide (LPS) of Klebsiella pneumoniae O1, a causative agent of lung infection, were identified by surface plasmon resonance. To characterize the molecular mechanism of this interaction, HL binding to synthetic disaccharides and tetrasaccharides representing one and two repeating units, respectively, of the O-chain of this LPS were studied. pH-dependent structural rearrangements of HL after interaction with the disaccharide were observed through nuclear magnetic resonance. The crystal structure of the HL-tetrasaccharide complex revealed carbohydrate chain packing into the A, B, C, and D binding sites of HL, which primarily occurred through residue-specific, direct or water-mediated hydrogen bonds and hydrophobic contacts. Overall, these results support a crucial role of the Glu35/Asp53/Trp63/Asp102 residues in HL binding to the tetrasaccharide. These observations suggest an unknown glycan-guided mechanism that underlies recognition of the bacterial cell wall by lysozyme and may complement the HL immune defense function.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- INTO-FURANOSIDE REARRANGEMENT
- EGG-WHITE LYSOZYME
- KLEBSIELLA-PNEUMONIAE
- RECOGNITION
- REFINEMENT
- PROGRAM
- BINDING
- NMR
- HEN
- LIPOPOLYSACCHARIDE
Publication and Content Type
- ref (subject category)
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- By the author/editor
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Zhang, R. Y.
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Wu, L. S.
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Eckert, T.
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Burg-Roderfeld, ...
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Rojas-Macias, M. ...
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Lutteke, T.
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show more...
-
Krylov, V. B.
-
Argunov, D. A.
-
Datta, A.
-
Markart, P.
-
Guenther, A.
-
Norden, B.
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Schauer, R.
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Bhunia, A.
-
Enani, M. A.
-
Billeter, M.
-
Scheidig, A. J.
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Nifantiev, Nikol ...
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Siebert, H. C.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
- Articles in the publication
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Quarterly Review ...
- By the university
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University of Gothenburg