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Serial femtosecond ...
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Andersson, Rebecka,1988Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
(author)
Serial femtosecond crystallography structure of cytochrome c oxidase at room temperature.
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2017-07-03
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Springer Science and Business Media LLC,2017
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/255358
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https://gup.ub.gu.se/publication/255358URI
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https://doi.org/10.1038/s41598-017-04817-zDOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Cytochrome c oxidase catalyses the reduction of molecular oxygen to water while the energy released in this process is used to pump protons across a biological membrane. Although an extremely well-studied biological system, the molecular mechanism of proton pumping by cytochrome c oxidase is still not understood. Here we report a method to produce large quantities of highly diffracting microcrystals of ba 3-type cytochrome c oxidase from Thermus thermophilus suitable for serial femtosecond crystallography. The room-temperature structure of cytochrome c oxidase is solved to 2.3Å resolution from data collected at an X-ray Free Electron Laser. We find overall agreement with earlier X-ray structures solved from diffraction data collected at cryogenic temperature. Previous structures solved from synchrotron radiation data, however, have shown conflicting results regarding the identity of the active-site ligand. Our room-temperature structure, which is free from the effects of radiation damage, reveals that a single-oxygen species in the form of a water molecule or hydroxide ion is bound in the active site. Structural differences between the ba 3-type and aa 3-type cytochrome c oxidases around the proton-loading site are also described.
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Safari, Cecilia,1989Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xsafce
(author)
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Dods, Robert,1989Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xdodsr
(author)
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Nango, Eriko
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Tanaka, Rie
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Yamashita, Ayumi
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Nakane, Takanori
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Tono, Kensuke
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Joti, Yasumasa
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Båth, Petra,1988Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xbatpe
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Dunevall, Elin,1986Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xjelix
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Bosman, Robert,1991Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xbosro
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Nureki, Osamu
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Iwata, So
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Neutze, Richard,1969Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xneuri
(author)
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Brändén, Gisela,1975Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xbragi
(author)
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Göteborgs universitetInstitutionen för kemi och molekylärbiologi
(creator_code:org_t)
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In:Scientific reports: Springer Science and Business Media LLC7:12045-2322
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Andersson, Rebec ...
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Safari, Cecilia, ...
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Dods, Robert, 19 ...
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Nango, Eriko
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Tanaka, Rie
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Yamashita, Ayumi
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Nakane, Takanori
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Tono, Kensuke
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Joti, Yasumasa
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Dunevall, Elin, ...
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Bosman, Robert, ...
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Nureki, Osamu
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Iwata, So
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