Mycophenolate mofetil ameliorates perivascular T lymphocyte inflammation and reduces the double-negative T cell population in SLE-prone MRLlpr/lpr mice.

Jonsson, Charlotte A,1971Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning,Institute of Internal Medicine, Dept of Rheumatology and Inflammation Research (author)

Effects on T lymphocyte mediated pathology, phenotypes, and functions in MRLlpr/lpr mice given mycophenolate mofetil (MMF) (100 mg/kg/day) via drinking water or controls given ip cyclophosphamide (CYC) injections (1.8 mg/mouse/week) or water were described. Both MMF and CYC treatment diminished kidney and large salivary gland perivascular cell infiltrates, reduced profoundly double-negative (DN) T cell frequencies, decreased total lymphocyte number in spleen, and increased in vitro proliferative response to Con A. IFN-gamma and IL-10 in supernatants from Con A stimulated spleen cells were augmented after MMF but not CYC treatment. MMF treatment increased whereas CYC reduced IL-12 in serum. Kidney expressions of IFN-gamma, IL-10, and IL-12 mRNA were unaffected by MMF but decreased by CYC. Our results demonstrate that MMF and CYC suppress perivascular T lymphocyte inflammation by reducing the DN T cell population and by amelioration of T cell function. The varying cytokine patterns suggest different mechanisms of the two drugs.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine hsv//eng
Animals
Anti-Inflammatory Agents
Non-Steroidal
pharmacology
B-Lymphocytes
drug effects
immunology
Cell Count
Cell Division
Concanavalin A
pharmacology
Cyclophosphamide
pharmacology
Cytokines
blood
genetics
Disease Models
Animal
Female
Hypersensitivity
Delayed
Immunophenotyping
Immunosuppressive Agents
pharmacology
Kidney
blood supply
cytology
immunology
Lupus Erythematosus
Systemic
immunology
Male
Mice
Mice
Inbred MRL lpr
Mycophenolic Acid
analogs & derivatives
pharmacology
RNA
Messenger
Salivary Glands
blood supply
cytology
Spleen
cytology
immunology
T-Lymphocytes
cytology
drug effects
immunology
Vasculitis
pathology

Erlandsson, Malin,1972Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning,Institute of Internal Medicine, Dept of Rheumatology and Inflammation Research(Swepub:gu)xerlma (author)
Svensson, Lena,1958Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning,Institute of Internal Medicine, Dept of Rheumatology and Inflammation Research (author)
Mölne, J (author)
Carlsten, Hans,1954Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning,Institute of Internal Medicine, Dept of Rheumatology and Inflammation Research(Swepub:gu)xcahan (author)
Göteborgs universitetInstitutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning (creator_code:org_t)

By the author/editor: Jonsson, Charlot ...; Erlandsson, Mali ...; Svensson, Lena, ...; Mölne, J; Carlsten, Hans, ...

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