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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04561naa a2200685 4500 | |
| 001 | oai:gup.ub.gu.se/257351 | |
| 003 | SwePub | |
| 008 | 240528s1998 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://gup.ub.gu.se/publication/2573512 URI |
| 024 | 7 | a https://doi.org/10.1210/jcem.83.8.50172 DOI |
| 040 | a (SwePub)gu | |
| 041 | a eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Boguszewski, C L4 aut |
| 245 | 1 0 | a Cloning of two novel growth hormone transcripts expressed in human placenta. |
| 264 | 1 | b The Endocrine Society,c 1998 |
| 520 | a Several isoforms of human GH (hGH) are produced by two related genes expressed in the pituitary (hGH-N) and in the placenta (hGH-V). These genes consist of five exons (denoted 1-5) separated by four introns (denoted A-D). In the present report, two new transcripts of the hGH-V gene are described. The coding region of the hGH-V gene was amplified by RT-PCR using placental complementary DNA as template. DNA sequencing of several clones revealed two novel transcripts. One had a 45-bp deletion caused by the use of an alternative splice acceptor site within exon 3, similar to that in the hGH-N gene, predicting a 20-kDa isoform of hGH-V. The other transcript was generated by the use of an alternative splice donor site causing a 4-bp deletion in the end of exon 4, predicting a 24-kDa protein with 219 amino acids, which we refer to as hGH-V3. The carboxy-terminal sequence of hGH-V3 differs from 22-kDa hGH-V and hGH-V2, the two previously reported transcripts of the hGH-V gene, and does not contain a predicted transmembrane domain as described for hGH-V2. Ligase chain reaction was then used to analyze the possible use of the same splicing pattern in transcripts derived from the other genes of the hGH-gene cluster. Alternatively spliced transcripts encoding the 20-kDa hGH isoform were detected from the hGH-N and hGH-V genes, but not from the human chorionic somatomammotropin-A/B genes. The alternative splicing generating hGH-V3 was only demonstrated in transcripts derived from the hGH-V gene. Using competitive RT-PCR, the expression of hGH-V3 was estimated to be 10% of the hGH-V messenger RNA in full-term normal placentas and in placentas from pathological pregnancies. The 20-kDa hGH-V was detected in two of four full-term normal placentas, whereas a weak signal was observed in one of the pathological placentas. We conclude that the hGH-V primary transcript undergoes alternative splicing pathways generating at least four different messenger RNAs, predicting the expression of different hGH isoforms, including two with a complete sequence divergence in the carboxy-terminus. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaper0 (SwePub)3012 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicine0 (SwePub)3012 hsv//eng |
| 653 | a Alternative Splicing | |
| 653 | a Amino Acid Sequence | |
| 653 | a Base Sequence | |
| 653 | a Chemical Phenomena | |
| 653 | a Chemistry | |
| 653 | a Physical | |
| 653 | a Cloning | |
| 653 | a Molecular | |
| 653 | a DNA | |
| 653 | a Complementary | |
| 653 | a Female | |
| 653 | a Gene Expression | |
| 653 | a Genetic Variation | |
| 653 | a Human Growth Hormone | |
| 653 | a chemistry | |
| 653 | a genetics | |
| 653 | a Humans | |
| 653 | a Molecular Sequence Data | |
| 653 | a Placenta | |
| 653 | a chemistry | |
| 653 | a Polymerase Chain Reaction | |
| 653 | a Pregnancy | |
| 653 | a RNA | |
| 653 | a Messenger | |
| 653 | a analysis | |
| 653 | a genetics | |
| 653 | a RNA-Directed DNA Polymerase | |
| 653 | a Sequence Analysis | |
| 653 | a DNA | |
| 700 | 1 | a Svensson, Per-Arne,d 1969u Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine4 aut0 (Swepub:gu)xsvper |
| 700 | 1 | a Jansson, T4 aut |
| 700 | 1 | a Clark, R4 aut |
| 700 | 1 | a Carlsson, L M4 aut |
| 700 | 1 | a Carlsson, B4 aut |
| 710 | 2 | a Göteborgs universitetb Institutionen för invärtesmedicin, Avdelningen för internmedicin4 org |
| 773 | 0 | t The Journal of clinical endocrinology and metabolismd : The Endocrine Societyg 83:8, s. 2878-85q 83:8<2878-85x 0021-972Xx 1945-7197 |
| 856 | 4 | u https://academic.oup.com/jcem/article-pdf/83/8/2878/10403955/jcem2878.pdf |
| 856 | 4 8 | u https://gup.ub.gu.se/publication/257351 |
| 856 | 4 8 | u https://doi.org/10.1210/jcem.83.8.5017 |
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