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High resolution X-r...
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Jacobson, Frida,1975Chalmers tekniska högskola,Chalmers University of Technology
(author)
High resolution X-ray structures of the oxidised and reduced forms of nitrite reductase from Rhodobacter sphaeroides 2.4.3
- Article/chapterEnglish2005
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LIBRIS-ID:oai:gup.ub.gu.se/25762
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https://gup.ub.gu.se/publication/25762URI
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https://doi.org/10.1107/S0907444905017488DOI
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https://research.chalmers.se/publication/25762URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Nitrite reductase is an enzyme operating in the denitrification pathway which catalyses the conversion of nitrite (NO2(-)) to gaseous nitric oxide (NO). Here, crystal structures of the oxidized and reduced forms of the copper-containing nitrite reductase from Rhodobacter sphaeroides 2.4.3 are presented at 1.74 and 1.85 A resolution, respectively. Whereas the structure of the enzyme is very similar to those of other copper-containing nitrite reductases, folding as a trimer and containing two copper sites per monomer, the structures reported here enable conformational differences between the oxidized and reduced forms of the enzyme to be identified. In the type 1 copper site, a rotational perturbation of the side chain of the copper ligand Met182 occurs upon reduction. At the type 2 copper site, a dual conformation of the catalytic residue His287 is observed in the oxidized structure but is lacking in the reduced structure, such that the interactions of the oxidized type 2 copper ion can be regarded as adopting octahedral geometry. These findings shed light on the structural mechanism of the reduction of a copper-bound nitrite to nitric oxide and water.
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Guo, KAstraZeneca AB
(author)
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Olesen, Kenneth,1966Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg(Swepub:gu)xoleke
(author)
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Ökvist, Mats,1970Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
(author)
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Neutze, Richard,1969Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)xneuri
(author)
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Sjölin, Lennart,1949Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xsjoll
(author)
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Chalmers tekniska högskolaAstraZeneca AB
(creator_code:org_t)
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In:Acta Crystallography D61, s. 1190-11980907-44491399-0047
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Jacobson, Frida, ...
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Guo, K
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- NATURAL SCIENCES
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Acta Crystallogr ...
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University of Gothenburg
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Chalmers University of Technology