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High resolution X-r...
High resolution X-ray structures of the oxidised and reduced forms of nitrite reductase from Rhodobacter sphaeroides 2.4.3
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- Jacobson, Frida, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Guo, K (author)
- AstraZeneca AB
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- Olesen, Kenneth, 1966 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry,University of Gothenburg
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- Ökvist, Mats, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Neutze, Richard, 1969 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Sjölin, Lennart, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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(creator_code:org_t)
- 2005
- 2005
- English.
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In: Acta Crystallography D. - 0907-4449 .- 1399-0047. ; 61, s. 1190-1198
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Abstract
Subject headings
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- Nitrite reductase is an enzyme operating in the denitrification pathway which catalyses the conversion of nitrite (NO2(-)) to gaseous nitric oxide (NO). Here, crystal structures of the oxidized and reduced forms of the copper-containing nitrite reductase from Rhodobacter sphaeroides 2.4.3 are presented at 1.74 and 1.85 A resolution, respectively. Whereas the structure of the enzyme is very similar to those of other copper-containing nitrite reductases, folding as a trimer and containing two copper sites per monomer, the structures reported here enable conformational differences between the oxidized and reduced forms of the enzyme to be identified. In the type 1 copper site, a rotational perturbation of the side chain of the copper ligand Met182 occurs upon reduction. At the type 2 copper site, a dual conformation of the catalytic residue His287 is observed in the oxidized structure but is lacking in the reduced structure, such that the interactions of the oxidized type 2 copper ion can be regarded as adopting octahedral geometry. These findings shed light on the structural mechanism of the reduction of a copper-bound nitrite to nitric oxide and water.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Copper
- chemistry
- Crystallography
- X-Ray
- Hydrogen-Ion Concentration
- Nitrite Reductases
- chemistry
- metabolism
- Oxidation-Reduction
- Protein Conformation
- Rhodobacter sphaeroides
- enzymology
- Rhodobacter sphaeroides
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Jacobson, Frida, ...
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Guo, K
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Olesen, Kenneth, ...
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Ökvist, Mats, 19 ...
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Neutze, Richard, ...
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Sjölin, Lennart, ...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Acta Crystallogr ...
- By the university
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University of Gothenburg
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Chalmers University of Technology