QKI6B mRNA levels are upregulated in schizophrenia and predict GFAP expression

Farnsworth, B.,1987-Uppsala universitet,Evolution och utvecklingsbiologi (author)

Schizophrenia is a highly heritable disorder with a heterogeneous symptomatology. Research increasingly indicates the importance of the crucial and often overlooked glial perturbations within schizophrenia. Within this study, we examined an isoform of quaking (a gene encoding an RNA-binding protein that is exclusively expressed in glial cells), known as QKI6B, and a prototypical astrocyte marker, glial fibrillary acidic protein (GFAP), postulated to be under the regulation of QKI. The expression levels of these genes were quantified across post-mortem brain samples from 55 schizophrenic individuals, and 55 healthy controls, using real-time PCR. We report, through an analysis of covariance (ANCOVA) model, an upregulation of both QKI6B, and GFAP in the prefrontal cortex of brain samples of schizophrenic individuals, as compared to control samples. Previous research has suggested that the QKI protein directly regulates the expression of several genes through interaction with a motif in the target's sequence, termed the Quaking Response Element (QRE). We therefore examined if QICI6B expression can predict the outcome of GFAP, and several oligodendrocyte-related genes, using a multiple linear regression approach. We found that QKI6B significantly predicts the expression of GFAP, but does not predict oligodendrocyte-related gene outcome, as previously seen with other QKI isoforms.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Neurologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Neurology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
Schizophrenia
Glia
Astrocytes
qPCR
ANCOVA
Regression
fibrillary-acidic-protein
anterior cingulate cortex
prefrontal cortex
bipolar disorder
glutamine-synthetase
subgenual cingulate
synaptic
proteins
serine racemase
frontal-cortex
star protein
Neurosciences & Neurology
Schizophrenia

Radomska, K.J.1984-Uppsala universitet,Evolution och utvecklingsbiologi,IBENS, Dept Biol, Paris, France.(Swepub:uu)katra417 (author)
Zimmermann, B.Uppsala universitet,Evolution och utvecklingsbiologi (author)
Kettunen, PetronellaGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,Univ Gothenburg, Sahlgrenska Acad, Dept Psychiat & Neurochem, Inst Neurosci & Physiol, Gothenburg, Sweden.;Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Neurosci, Dept Neuropathol, Oxford, England.(Swepub:gu)xketpe (author)
Jazin, E.Uppsala universitet,Evolution och utvecklingsbiologi(Swepub:uu)elenjazi (author)
Emilsson, L.S.Uppsala universitet,Evolution och utvecklingsbiologi(Swepub:uu)linaemil (author)
Uppsala universitetEvolution och utvecklingsbiologi (creator_code:org_t)

By the author/editor: Farnsworth, B., ...; Radomska, K.J. 1 ...; Zimmermann, B.; Kettunen, Petron ...; Jazin, E.; Emilsson, L.S.

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Neurology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Neurosciences

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