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Binding Kinetics an...
Binding Kinetics and Lateral Mobility of HSV-1 on End-Grafted Sulfated Glycosaminoglycans
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- Peerboom, Nadia, 1990 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Block, Stephan, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Altgärde, Noomi, 1983 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Wahlsten, Olov, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Moller, S. (author)
- Innovent e.V.
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- Schnabelrauch, M. (author)
- Innovent e.V.
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- Trybala, Edward, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg
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- Bergström, Tomas, 1950 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg
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- Bally, Marta, 1981 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Chalmers tekniska högskola Innovent eV. (creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English.
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In: Biophysical Journal. - : Elsevier BV. - 0006-3495 .- 1542-0086. ; 113:6, s. 1223-1234
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Abstract
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- Many viruses, including herpes simplex (HSV), are recruited to their host cells via interaction between their envelope glycoproteins and cell-surface glycosaminoglycans (GAGs). This initial attachment is of a multivalent nature, i.e., it requires the establishment of multiple bonds between amino acids of viral glycoproteins and sulfated saccharides on the GAG chain. To gain understanding of how this binding process is modulated, we performed binding kinetics and mobility studies using end-grafted GAG chains that mimic the end attachment of these chains to proteoglycans. Total internal reflection fluorescence microscopy was used to probe binding and release, as well as the diffusion of single HSV-1 particles. To verify the hypothesis that the degree of sulfation, but also the arrangement of sulfate groups along the GAG chain, plays a key role in HSV binding, we tested two native GAGs (chondroitin sulfate and heparan sulfate) and compared our results to chemically sulfated hyaluronan. HSV-1 recognized all sulfated GAGs, but not the nonsulfated hyaluronan, indicating that binding is specific to the presence of sulfate groups. Furthermore we observed that a notable fraction of GAG-bound virions exhibit lateral mobility, although the multivalent binding to the immobilized GAG brushes ensures firm virus attachment to the interface. Diffusion was faster on the two native GAGs, one of which, chondroitin sulfate, was also characterized by the highest association rate per GAG chain. This highlights the complexity of multivalent virus-GAG interactions and suggests that the spatial arrangement of sulfates along native GAG chains may play a role in modulating the characteristics of the HSV-GAG interaction. Altogether, these results, obtained with a minimal and well-controlled model of the cell membrane, provide, to our knowledge, new insights into the dynamics of the HSV-GAG interaction.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- herpes-simplex-virus
- surface-plasmon resonance
- single-particle
- tracking
- type-1 glycoprotein-c
- heparan-sulfate
- chondroitin sulfate
- lipid-membrane
- live cells
- protein
- attachment
- Biophysics
- feijter ja
- 1978
- biopolymers
- v17
- p1759
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Peerboom, Nadia, ...
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Block, Stephan, ...
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Altgärde, Noomi, ...
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Wahlsten, Olov, ...
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Moller, S.
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Schnabelrauch, M ...
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show more...
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Trybala, Edward, ...
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Bergström, Tomas ...
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Bally, Marta, 19 ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
- Articles in the publication
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Biophysical Jour ...
- By the university
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University of Gothenburg
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Chalmers University of Technology