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Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
- Kubo, M. (author)
- Nango, E. (author)
- Tono, K. (author)
- show more...
- Kimura, T. (author)
- Owada, S. (author)
- Song, C. Y. (author)
- Mafune, F. (author)
- Miyajima, K. (author)
- Takeda, Y. (author)
- Kohno, J. (author)
- Miyauchi, N. (author)
- Nakane, T. (author)
- Tanaka, T. (author)
- Nomura, T. (author)
- Davidsson, J. (author)
- Tanaka, R. (author)
- Murata, M. (author)
- Kameshima, T. (author)
- Hatsui, T. (author)
- Joti, Y. (author)
- Yabashi, M. (author)
- Iwata, S. (author)
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- (creator_code:org_t)
- 2017-08-22
- 2017
- English.
- In: Journal of Synchrotron Radiation. - : International Union of Crystallography (IUCr). - 1600-5775. ; 24, s. 1086-1091
- Journal article (peer-reviewed)
Abstract
Subject headings
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- X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
Subject headings
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- XFEL
- serial femtosecond crystallography
- time-resolved X-ray crystallography
- pump and probe
- free-electron laser
- protein-structure determination
- photoactive yellow
- protein
- x-ray-diffraction
- structural-changes
- bacteriorhodopsin
- Instruments & Instrumentation
- Optics
- Physics
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Kubo, M.
- Nango, E.
- Tono, K.
- Kimura, T.
- Owada, S.
- Song, C. Y.
- show more...
- Mafune, F.
- Miyajima, K.
- Takeda, Y.
- Kohno, J.
- Miyauchi, N.
- Nakane, T.
- Tanaka, T.
- Nomura, T.
- Davidsson, J.
- Tanaka, R.
- Murata, M.
- Kameshima, T.
- Hatsui, T.
- Joti, Y.
- Neutze, Richard, ...
- Yabashi, M.
- Iwata, S.
- show less...
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- University of Gothenburg
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