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Nanosecond pump-pro...
Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
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Kubo, M. (author)
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Nango, E. (author)
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Tono, K. (author)
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Kimura, T. (author)
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Owada, S. (author)
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Song, C. Y. (author)
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Mafune, F. (author)
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Miyajima, K. (author)
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Takeda, Y. (author)
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Kohno, J. (author)
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Miyauchi, N. (author)
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Nakane, T. (author)
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Tanaka, T. (author)
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Nomura, T. (author)
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Davidsson, J. (author)
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Tanaka, R. (author)
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Murata, M. (author)
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Kameshima, T. (author)
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Hatsui, T. (author)
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Joti, Y. (author)
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- Neutze, Richard, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Yabashi, M. (author)
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Iwata, S. (author)
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(creator_code:org_t)
- 2017-08-22
- 2017
- English.
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In: Journal of Synchrotron Radiation. - : International Union of Crystallography (IUCr). - 1600-5775. ; 24, s. 1086-1091
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
Subject headings
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- XFEL
- serial femtosecond crystallography
- time-resolved X-ray crystallography
- pump and probe
- free-electron laser
- protein-structure determination
- photoactive yellow
- protein
- x-ray-diffraction
- structural-changes
- bacteriorhodopsin
- Instruments & Instrumentation
- Optics
- Physics
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Kubo, M.
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Nango, E.
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Tono, K.
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Kimura, T.
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Owada, S.
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Song, C. Y.
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show more...
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Mafune, F.
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Miyajima, K.
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Takeda, Y.
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Kohno, J.
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Miyauchi, N.
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Nakane, T.
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Tanaka, T.
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Nomura, T.
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Davidsson, J.
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Tanaka, R.
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Murata, M.
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Kameshima, T.
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Hatsui, T.
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Joti, Y.
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Neutze, Richard, ...
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Yabashi, M.
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Iwata, S.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
- Articles in the publication
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Journal of Synch ...
- By the university
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University of Gothenburg