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Nanosecond pump-pro...
Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2017-08-22
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International Union of Crystallography (IUCr),2017
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/258808
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https://gup.ub.gu.se/publication/258808URI
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https://doi.org/10.1107/s160057751701030xDOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Nango, E.
(author)
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Tono, K.
(author)
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Kimura, T.
(author)
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Owada, S.
(author)
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Song, C. Y.
(author)
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Mafune, F.
(author)
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Miyajima, K.
(author)
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Takeda, Y.
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Kohno, J.
(author)
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Miyauchi, N.
(author)
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Nakane, T.
(author)
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Tanaka, T.
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Nomura, T.
(author)
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Davidsson, J.
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Tanaka, R.
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Murata, M.
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Kameshima, T.
(author)
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Hatsui, T.
(author)
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Joti, Y.
(author)
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Neutze, Richard,1969Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xneuri
(author)
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Yabashi, M.
(author)
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Iwata, S.
(author)
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Göteborgs universitetInstitutionen för kemi och molekylärbiologi
(creator_code:org_t)
Related titles
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In:Journal of Synchrotron Radiation: International Union of Crystallography (IUCr)24, s. 1086-10911600-5775
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Kubo, M.
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Nango, E.
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Tono, K.
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Kimura, T.
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Owada, S.
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Song, C. Y.
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show more...
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Mafune, F.
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Miyajima, K.
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Takeda, Y.
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Kohno, J.
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Miyauchi, N.
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Nakane, T.
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Tanaka, T.
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Nomura, T.
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Davidsson, J.
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Tanaka, R.
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Murata, M.
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Kameshima, T.
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Hatsui, T.
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Joti, Y.
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Neutze, Richard, ...
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Yabashi, M.
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Iwata, S.
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- NATURAL SCIENCES
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NATURAL SCIENCES
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Journal of Synch ...
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University of Gothenburg