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Transfer and Persis...
Transfer and Persistence of a Multi-Drug Resistance Plasmid in situ of the Infant Gut Microbiotain the Absence of Antibiotic Treatment
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- Gumpert, H. (author)
- Tech Univ Denmark, Dept Syst Biol, Lyngby, Denmark.;Univ Copenhagen, Hvidovre Hosp, Dept Clin Microbiol, Hvidovre, Denmark.
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- Kubicek-Sutherland, J. Z. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Porse, A. (author)
- Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, Lyngby, Denmark.
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- Karami, Nahid, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Infect Dis, Gothenburg, Sweden.
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- Munck, C. (author)
- Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, Lyngby, Denmark.
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- Linkevicius, Marius (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Adlerberth, Ingegerd, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Infect Dis, Gothenburg, Sweden.
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- Wold, Agnes E, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Infect Dis, Gothenburg, Sweden.
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- Andersson, Dan I. (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Sommer, M. O. A. (author)
- Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, Lyngby, Denmark.
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Tech Univ Denmark, Dept Syst Biol, Lyngby, Denmark;Univ Copenhagen, Hvidovre Hosp, Dept Clin Microbiol, Hvidovre, Denmark. Institutionen för medicinsk biokemi och mikrobiologi (creator_code:org_t)
- 2017-09-26
- 2017
- English.
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In: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 8
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Abstract
Subject headings
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- The microbial ecosystem residing in the human gut is believed to play an important role in horizontal exchange of virulence and antibiotic resistance genes that threatens human health. While the diversity of gut-microorganisms and their genetic content has been studied extensively, high-resolution insight into the plasticity, and selective forces shaping individual genomes is scarce. In a longitudinal study, we followed the dynamics of co-existing Escherichia coli lineages in an infant not receiving antibiotics. Using whole genome sequencing, we observed large genomic deletions, bacteriophage infections, as well as the loss and acquisition of plasmids in these lineages during their colonization of the human gut. In particular, we captured the exchange of multidrug resistance genes, and identified a clinically relevant conjugative plasmid mediating the transfer. This resistant transconjugant lineage was maintained for months, demonstrating that antibiotic resistance genes can disseminate and persist in the gut microbiome; even in absence of antibiotic selection. Furthermore, through in vivo competition assays, we suggest that the resistant transconjugant can persist through a fitness advantage in the mouse gut in spite of a fitness cost in vitro. Our findings highlight the dynamic nature of the human gut microbiota and provide the first genomic description of antibiotic resistance gene transfer between bacteria in the unperturbed human gut. These results exemplify that conjugative plasmids, harboring resistance determinants, can transfer and persists in the gut in the absence of antibiotic treatment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Escherichia coli
- horizontal gene transfer
- infant gut
- genome dynamics
- plasmid transfer
- in vivo
- escherichia-coli
- ampicillin resistance
- klebsiella-pneumoniae
- mouse
- intestine
- gene-transfer
- vivo transfer
- sequence
- colonization
- virulence
- elements
- Microbiology
- CONNELL M A
- 1991
- Microbial Ecology in Health and Disease
- V4
- P343
- Escherichia coli
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Gumpert, H.
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Kubicek-Sutherla ...
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Porse, A.
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Karami, Nahid, 1 ...
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Munck, C.
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Linkevicius, Mar ...
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Adlerberth, Inge ...
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Wold, Agnes E, 1 ...
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Andersson, Dan I ...
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Sommer, M. O. A.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Frontiers in Mic ...
- By the university
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University of Gothenburg
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Uppsala University