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Lubricin binds cart...
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Flowers, Sarah A.University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Med Biochem & Cell Biol, Gothenburg, Sweden.
(author)
Lubricin binds cartilage proteins, cartilage oligomeric matrix protein, fibronectin and collagen II at the cartilage surface
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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2017-10-13
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Springer Science and Business Media LLC,2017
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/259565
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https://gup.ub.gu.se/publication/259565URI
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https://doi.org/10.1038/s41598-017-13558-yDOI
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https://lup.lub.lu.se/record/ea36cf1f-9114-4152-90a6-2c0599f9f0abURI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:136848874URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-337332URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Lubricin, a heavily O-glycosylated protein, is essential for boundary lubrication of articular cartilage. Strong surface adherence of lubricin is required given the extreme force it must withstand. Disulfide bound complexes of lubricin and cartilage oligomeric matrix protein (COMP) have recently been identified in arthritic synovial fluid suggesting they may be lost from the cartilage surface in osteoarthritis and inflammatory arthritis. This investigation was undertaken to localise COMP-lubricin complexes within cartilage and investigate if other cartilage proteins are involved in anchoring lubricin to the joint. Immunohistochemical analysis of human cartilage biopsies showed lubricin and COMP co-localise to the cartilage surface. COMP knockout mice, however, presented with a lubricin layer on the articular cartilage leading to the further investigation of additional lubricin binding mechanisms. Proximity ligation assays (PLA) on human cartilage biopsies was used to localise additional lubricin binding partners and demonstrated that lubricin bound COMP, but also fibronectin and collagen II on the cartilage surface. Fibronectin and collagen II binding to lubricin was confirmed and characterised by solid phase binding assays with recombinant lubricin fragments. Overall, COMP, fibronectin and collagen II bind lubricin, exposed on the articular cartilage surface suggesting they may be involved in maintaining essential boundary lubrication.
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Zieba, AgataUppsala universitet,Uppsala University,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi(Swepub:uu)agazi628
(author)
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Örnros, JessicaUniversity of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Med Biochem & Cell Biol, Gothenburg, Sweden.
(author)
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Jin, ChunshengUniversity of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Med Biochem & Cell Biol, Gothenburg, Sweden.(Swepub:gu)xjinch
(author)
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Rolfson, Ola,1973University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för ortopedi,Institute of Clinical Sciences, Department of Orthopaedics,Univ Gothenburg, Sahlgrenska Acad, Inst Clin Sci, Dept Orthopaed, Gothenburg, Sweden.(Swepub:gu)xrolol
(author)
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Björkman, Lena,1965University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research,Univ Gothenburg, Sahlgrenska Acad, Inst Med, Dept Rheumatol & Inflammat Res, Gothenburg, Sweden.(Swepub:gu)xblenv
(author)
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Eisler, T.Karolinska Institutet,Karolinska Institute,Danderyd Hospital,Karolinska Inst, Danderyd Hosp, Dept Clin Sci, Stockholm, Sweden.
(author)
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Kalamajski, SebastianLund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups,Lund Univ, Dept Mol Skeletal Biol, Lund, Sweden.(Swepub:lu)se8335ka
(author)
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Kamali-Moghaddam, MasoodUppsala universitet,Uppsala University,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi(Swepub:uu)masookam
(author)
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Karlsson, Niclas G.,1966University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Med Biochem & Cell Biol, Gothenburg, Sweden.(Swepub:gu)xkanic
(author)
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
(creator_code:org_t)
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Zieba, Agata
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Örnros, Jessica
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Eisler, T.
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