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Selective release o...
Selective release of miRNAs via extracellular vesicles is associated with house-dust mite allergen-induced airway inflammation
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Gon, Y. (author)
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Maruoka, S. (author)
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Inoue, T. (author)
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Kuroda, K. (author)
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Yamagishi, K. (author)
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Kozu, Y. (author)
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Shikano, S. (author)
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Soda, K. (author)
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- Lötvall, Jan, 1956 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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Hashimoto, S. (author)
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(creator_code:org_t)
- 2017-11-29
- 2017
- English.
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In: Clinical and Experimental Allergy. - : Wiley. - 0954-7894. ; 47:12, s. 1586-1598
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Abstract
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- Background: MicroRNAs (miRNAs) may facilitate cell-to-cell communication via extracellular vesicles (EVs). The biological roles of miRNAs in EVs on allergic airway inflammation are unclear. Methods: Airway-secreted EVs (AEVs) were isolated from bronchoalveolar lavage fluid (BALF) of control and house-dust mite (HDM) allergen-exposed HDM-sensitized mice. The expression of miRNAs in AEVs or miRNAs and mRNAs in lung tissue was analysed using miRNA microarray. Results: The amount of AEV increased 8.9-fold in BALF from HDM-exposed mice compared with that from sham-control mice. HDM exposure resulted in significant changes in the expression of 139 miRNAs in EVs and 175 miRNAs in lung tissues, with 54 miRNAs being common in both samples. Expression changes of these 54 miRNAs between miRNAs in AEVs and lung tissues after HDM exposure were inversely correlated. Computational analysis revealed that 31 genes, including IL-13 and IL-5Ra, are putative targets of the miRNAs up-regulated in AEVs but downregulated in lung tissues after HDM exposure. The amount of AEV in BALF after HDM exposure was diminished by treatment with the sphingomyelinase inhibitor GW4869. The treatment with GW4869 also decreased Th2 cytokines and eosinophil counts in BALFs and reduced eosinophil accumulation in airway walls and mucosa. Conclusion: These results indicate that selective sorting of miRNA including Th2 inhibitory miRNAs into AEVs and increase release to the airway after HDM exposure would be involved in the pathogenesis of allergic airway inflammation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- allergen
- asthma
- exosomes
- extracellular vesicles
- house-dust mite
- microvesicles
- miRNA
- Th2
- endothelial growth-factor
- fluid-phase measurements
- c-reactive protein
- occupational rhinitis
- nasal lavage
- inhalation challenge
- haptoglobin
- level
- asthma
- expression
- adhesion
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Gon, Y.
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Maruoka, S.
-
Inoue, T.
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Kuroda, K.
-
Yamagishi, K.
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Kozu, Y.
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show more...
-
Shikano, S.
-
Soda, K.
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Lötvall, Jan, 19 ...
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Hashimoto, S.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Clinical and Exp ...
- By the university
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University of Gothenburg