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Liver-Kidney-on-Chi...
Liver-Kidney-on-Chip To Study Toxicity of Drug Metabolites
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Theobald, J. (author)
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Ghanem, A. (author)
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Wallisch, P. (author)
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- Banaeiyan, Amin A (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
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Andrade-Navarro, M. A. (author)
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Taskova, K. (author)
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Haltmeier, M. (author)
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Kurtz, A. (author)
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Becker, H. (author)
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Reuter, S. (author)
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Mrowka, R. (author)
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Cheng, X. L. (author)
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Wolfl, S. (author)
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(creator_code:org_t)
- 2017-12-04
- 2018
- English.
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In: Acs Biomaterials Science & Engineering. - : American Chemical Society (ACS). - 2373-9878. ; 4:1, s. 78-89
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Abstract
Subject headings
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- Advances in organ-on-chip technologies for the application in in vitro drug development provide an attractive alternative approach to replace ethically controversial animal testing and to establish a basis for accelerated drug development. In recent years, various chip-based tissue culture systems have been developed, which are mostly optimized for cultivation of one single cell type or organoid structure and lack the representation of multi organ interactions. Here we present an optimized microfluidic chip design consisting of interconnected compartments, which provides the possibility to mimic the exchange between different organ specific cell types and enables to study interdependent cellular responses between organs and demonstrate that such tandem system can greatly improve the reproducibility and efficiency of toxicity studies. In a simplified liver-kidney-on-chip model, we showed that hepatic cells that grow in microfluidic conditions abundantly and stably expressed metabolism-related biomarkers. Moreover, we applied this system for investigating the biotransformation and toxicity of Aflatoxin B1 (AFB1) and Benzoalphapyrene (BaP), as well as the interaction with other chemicals. The results clearly demonstrate that the toxicity and metabolic response to drugs can be evaluated in a flow-dependent manner within our system, supporting the importance of advanced interconnected multiorgans in microfluidic devices for application in in vitro toxicity testing and as optimized tissue culture systems for in vitro drug screening.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- organ-on-chip
- liver-on-chip
- kidney-on-chip
- toxicity
- drug metabolism
- microfluidic
- HepG2
- 3d human liver
- primary human hepatocytes
- a-chip
- cytotoxicity assay
- epithelial-cells
- dna-adducts
- hepg2 cells
- culture
- nephrotoxicity
- cancer
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Theobald, J.
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Ghanem, A.
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Wallisch, P.
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Banaeiyan, Amin ...
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Andrade-Navarro, ...
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Taskova, K.
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show more...
-
Haltmeier, M.
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Kurtz, A.
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Becker, H.
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Reuter, S.
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Mrowka, R.
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Cheng, X. L.
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Wolfl, S.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomaterials Sci ...
- Articles in the publication
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Acs Biomaterials ...
- By the university
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University of Gothenburg