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Monitoring the rele...
Monitoring the release of a NO photodonor from polymer nanoparticles: Via Förster resonance energy transfer and two-photon fluorescence imaging
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Conte, C. (author)
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Fraix, A. (author)
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- Thomsen, Hanna, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Ungaro, F. (author)
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Cardile, V. (author)
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Graziano, A. C. E. (author)
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- Ericson, Marica B, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Quaglia, F. (author)
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Sortino, S. (author)
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(creator_code:org_t)
- 2018
- 2018
- English.
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In: Journal of Materials Chemistry B. - : Royal Society of Chemistry (RSC). - 2050-7518 .- 2050-750X. ; 6:2, s. 249-256
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Abstract
Subject headings
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- Core-shell polymeric nanoparticles (NPs) made of either di-block or tri-block poly-ϵ-caprolactone and polyethylene glycol copolymers, covalently integrating Rhodamine B in the core or the shell have been prepared and a green fluorescent NO photodonor entrapped therein. One- and two-photon fluorescence experiments demonstrate that effective Förster Resonance Energy Transfer (FRET) occurs exclusively in the di-block NPs having the Rhodamine in the core, accounting for a localization of the NO photoreleaser in the inner part of the polymeric nanocarrier. These di-block NPs are stable in the presence of human serum albumin and their cargo release NO under exclusive excitation with visible light. Two-photon imaging experiments carried out using 900 nm NIR light, demonstrate that the release of the NO photodonor can be monitored in biological tissue, herein human skin, and provide insights into the integrity and penetration depth of the NPs. Toxicity experiments performed on NCTC keratinocyte cell lines in the dark and upon visible light irradiation show good biocompatibility of the polymeric system that therefore has a great potential in light of the multifaceted therapeutic role of NO. © The Royal Society of Chemistry.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Biocompatibility
- Cell culture
- Dyes
- Energy transfer
- Fluorescence
- Functional polymers
- Light
- Nanoparticles
- Photodegradation
- Photons
- Biological tissues
- Human serum albumins
- Polymer nanoparticles
- Polymeric nanoparticles
- Resonance energy transfer
- Two photon fluorescence
- Two-photon fluorescence imaging
- Visible-light irradiation
- Polymers
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Conte, C.
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Fraix, A.
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Thomsen, Hanna, ...
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Ungaro, F.
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Cardile, V.
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Graziano, A. C. ...
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show more...
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Ericson, Marica ...
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Quaglia, F.
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Sortino, S.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Journal of Mater ...
- By the university
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University of Gothenburg