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Alcohols enhance th...
Alcohols enhance the rate of acetic acid diffusion in S. cerevisiae: biophysical mechanisms and implications for acetic acid tolerance
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- Lindahl, Lina, 1984 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Genheden, Samuel (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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- Faria-Oliveira, F. (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Allard, Stefan, 1968 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Eriksson, Leif A, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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- Olsson, Lisbeth, 1963 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Bettiga, Maurizio, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2018-01-01
- 2018
- English.
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In: Microbial Cell. - : Shared Science Publishers OG. - 2311-2638. ; 5:1, s. 42-55
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Abstract
Subject headings
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- Microbial cell factories with the ability to maintain high productivity in the presence of weak organic acids, such as acetic acid, are required in many industrial processes. For example, fermentation media derived from lignocellulosic biomass are rich in acetic acid and other weak acids. The rate of diffusional entry of acetic acid is one parameter determining the ability of microorganisms to tolerance the acid. The present study demonstrates that the rate of acetic acid diffusion in S. cerevisiae is strongly affected by the alcohols ethanol and n-butanol. Ethanol of 40 g/L and n-butanol of 8 g/L both caused a 65% increase in the rate of acetic acid diffusion, and higher alcohol concentrations caused even greater increases. Molecular dynamics simulations of membrane dynamics in the presence of alcohols demonstrated that the partitioning of alcohols to the head group region of the lipid bilayer causes a considerable increase in the membrane area, together with reduced membrane thickness and lipid order. These changes in physiochemical membrane properties lead to an increased number of water molecules in the membrane interior, providing biophysical mechanisms for the alcohol-induced increase in acetic acid diffusion rate. nbutanol affected S. cerevisiae and the cell membrane properties at lower concentrations than ethanol, due to greater and deeper partitioning in the membrane. This study demonstrates that the rate of acetic acid diffusion can be strongly affected by compounds that partition into the cell membrane, and highlights the need for considering interaction effects between compounds in the design of microbial processes.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- ethanol
- n-butanol
- lignocellulose
- inhibitors
- molecular dynamics simulations
- membrane
- molecular-dynamics simulations
- programmed cell-death
- saccharomyces-cerevisiae
- plasma-membrane
- ethanol tolerance
- stress-adaptation
- intracellular ph
- lipid-bilayers
- force-field
- weak
- acids
- Cell Biology
- shra p
- 1989
- applied microbiology and biotechnology
- v30
- p294
- molecular dynamics simulations
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Lindahl, Lina, 1 ...
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Genheden, Samuel
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Faria-Oliveira, ...
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Allard, Stefan, ...
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Eriksson, Leif A ...
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Olsson, Lisbeth, ...
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show more...
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Bettiga, Maurizi ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Microbial Cell
- By the university
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University of Gothenburg
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Chalmers University of Technology