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Assessing antigen s...
Assessing antigen specific HLA-DR plus antibody secreting cell (DR plus ASC) responses in whole blood in enteric infections using an ELISPOT technique
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Bhuiyan, T. R. (author)
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Hoq, M. R. (author)
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Nishat, N. S. (author)
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Al Mahbuba, D. (author)
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Rashu, R. (author)
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Islam, K. (author)
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Hossain, L. (author)
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Harris, J. B. (author)
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Ryan, E. T. (author)
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Calderwood, S. B. (author)
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- Svennerholm, Ann-Mari, 1947 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Qadri, F. (author)
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(creator_code:org_t)
- Elsevier BV, 2018
- 2018
- English.
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In: Microbes and Infection. - : Elsevier BV. - 1286-4579. ; 20:2, s. 122-129
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https://doi.org/10.1...
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Abstract
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- Antibody secreting cells (ASCs) generate antibodies in an antigen-specific manner as part of the adaptive immune response to infections, and these cells increase their surface expression of HLA-DR. We have studied this parameter (HLA-DR+ASC) in patients with recent diarrheal infection using immuno-magnetic cell sorting and an enzyme linked immunospot (ELISPOT) technique that requires only one milliliter of blood. We validated this approach in adult patients with cholera (n = 15) or ETEC diarrhea (n = 30) on days 2, 7 and 30 after showing clinical symptom at the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b) hospital in Dhaka, and we compared responses to age-matched healthy controls (n = 7). We found that HLA-DRthorn ASC (DR+ASC) responses specific both for T cell-dependent (cholera toxin B subunit), and T cell-independent (lipopolysaccharide) antigens were elevated at day 7 after showing clinical cholera symptom. Similarly, DR+ASCs were elevated against both heat-labile toxin and colonization factors following ETEC infection. We observed significant correlations between antigen-specific DR+ASC responses and antigen-specific, gut homing ASC and plasma antibody responses. This study demonstrates that a simple ELISPOT procedure allows determination of antigen-specific ASC responses using a small volume of whole blood following diarrhea. This technique may be particularly useful in studying DR+ASC responses in young children and infants, either following infection or vaccination. (c) 2017 The Authors. Published by Elsevier Masson SAS on behalf of Institut Pasteur.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- HLA-DR
- ASCs
- Immune response
- Cholera
- ETEC
- ELISPOT
- enterotoxigenic escherichia-coli
- colonization factors
- immune-responses
- monoclonal-antibodies
- travelers diarrhea
- forming-cells
- global burden
- expression
- molecules
- cholera
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Bhuiyan, T. R.
-
Hoq, M. R.
-
Nishat, N. S.
-
Al Mahbuba, D.
-
Rashu, R.
-
Islam, K.
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show more...
-
Hossain, L.
-
Harris, J. B.
-
Ryan, E. T.
-
Calderwood, S. B ...
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Svennerholm, Ann ...
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Qadri, F.
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show less...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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Microbes and Inf ...
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University of Gothenburg