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Targeting Mycobacte...
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Velasquez, L. N.
(author)
Targeting Mycobacterium tuberculosis Antigens to Dendritic Cells via the DC-Specific-ICAM3-Grabbing-Nonintegrin Receptor Induces Strong T-Helper 1 Immune Responses
- Article/chapterEnglish2018
Publisher, publication year, extent ...
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2018-03-09
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Frontiers Media SA,2018
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/265643
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https://gup.ub.gu.se/publication/265643URI
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https://doi.org/10.3389/fimmu.2018.00471DOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Tuberculosis remains a major global health problem and efforts to develop a more effective vaccine have been unsuccessful so far. Targeting antigens (Ags) to dendritic cells (DCs) in vivo has emerged as a new promising vaccine strategy. In this approach, Ags are delivered directly to DCs via antibodies that bind to endocytic cell-surface receptors. Here, we explored DC-specific-ICAM3-grabbing-nonintegrin (DC-SIGN) targeting as a potential vaccine against tuberculosis. For this, we made use of the hSIGN mouse model that expresses human DC-SIGN under the control of the murine CD11c promoter. We show that in vitro and in vivo delivery of anti-DC-SIGN antibodies conjugated to Ag85B and peptide 25 of Ag85B in combination with anti-CD40, the fungal cell wall component zymosan, and the cholera toxin-derived fusion protein CTA1-DD induces strong Ag-specific CD4(+) T-cell responses. Improved anti-mycobacterial immunity was accompanied by increased frequencies of Ag-specific IFN-gamma(+) IL-2(+) TNF-alpha(+) polyfunctional CD4(+) T cells in vaccinated mice compared with controls. Taken together, in this study we provide the proof of concept that the human DC-SIGN receptor can be efficiently exploited for vaccine purposes to promote immunity against mycobacterial infections.
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Stuve, P.
(author)
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Gentilini, M. V.
(author)
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Swallow, M.
(author)
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Bartel, J.
(author)
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Lycke, Nils Y,1954Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xlycni
(author)
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Barkan, D.
(author)
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Martina, M.
(author)
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Lujan, H. D.
(author)
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Kalay, H.
(author)
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van Kooyk, Y.
(author)
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Sparwasser, T. D.
(author)
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Berod, L.
(author)
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för mikrobiologi och immunologi
(creator_code:org_t)
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In:Frontiers in Immunology: Frontiers Media SA91664-3224
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Velasquez, L. N.
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Stuve, P.
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Gentilini, M. V.
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Swallow, M.
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Bartel, J.
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Lycke, Nils Y, 1 ...
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show more...
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Barkan, D.
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Martina, M.
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Lujan, H. D.
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Kalay, H.
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van Kooyk, Y.
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Sparwasser, T. D ...
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Berod, L.
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
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Frontiers in Imm ...
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University of Gothenburg