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Cell Membrane Deriv...
Cell Membrane Derived Platform To Study Virus Binding Kinetics and Diffusion with Single Particle Sensitivity
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- Peerboom, Nadia, 1990 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Schmidt, Eneas, 1991 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Trybala, Edward, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg
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- Block, Stephan, 1978 (author)
- Freie Universität Berlin
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- Bergström, Thomas (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg
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- Pace, Hudson, 1982 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Bally, Marta, 1981 (author)
- Umeå universitet,Avdelningen för virologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Bally
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(creator_code:org_t)
- 2018-04-24
- 2018
- English.
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In: Acs Infectious Diseases. - : American Chemical Society (ACS). - 2373-8227. ; 4:6, s. 944-953
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Abstract
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- Discovery and development of new antiviral therapies essentially rely on two key factors: an in-depth understanding of the mechanisms involved in viral infection and the development of fast and versatile drug screening platforms. To meet those demands, we present a biosensing platform to probe virus-cell membrane interactions on a single particle level. Our method is based on the formation of supported lipid bilayers from cell membrane material. Using total internal reflection fluorescence microscopy, we report the contribution of viral and cellular components to the interaction kinetics of herpes simplex virus type 1 with the cell membrane. Deletion of glycoprotein C (gC), the main viral attachment glycoprotein, or deletion of heparan sulfate, an attachment factor on the cell membrane, leads to an overall decrease in association of virions to the membrane and faster dissociation from the membrane. In addition to this, we perform binding inhibition studies using the antiviral compound heparin to estimate its IC50 value. Finally, single particle tracking is used to characterize the diffusive behavior of the virus particles on the supported lipid bilayers. Altogether, our results promote this platform as a complement to existing bioanalytical assays, being at the interface between simplified artificial membrane models and live cell experiments.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- virus-membrane interactions
- supported lipid bilayers
- herpes simplex virus
- binding kinetics
- single particle tracking
- biomimetic sensing platform
- herpes-simplex-virus
- supported lipid-bilayers
- type-1 glycoprotein-c
- heparan-sulfate
- protein mobility
- hiv-1 particles
- live cells
- infection
- receptor
- fusion
- Pharmacology & Pharmacy
- Infectious Diseases
- keuchi y
- 1992
- journal of biological chemistry
- v267
- p14685
- sai ur
- 1993
- biochemistry
- v32
- p8140
- single particle tracking
Publication and Content Type
- ref (subject category)
- art (subject category)
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Peerboom, Nadia, ...
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Schmidt, Eneas, ...
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Trybala, Edward, ...
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Block, Stephan, ...
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Bergström, Thoma ...
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Pace, Hudson, 19 ...
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Bally, Marta, 19 ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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Acs Infectious D ...
- By the university
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University of Gothenburg
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Chalmers University of Technology
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Umeå University