Components in Lentinus edodes mushroom with anti-biofilm activity directed against bacteria involved in caries and gingivitis

• The present study investigated the compounds present in the low molecular mass fraction of Lentinus edodes mushroom (shiitake) extract and their anti-virulence activity against oral pathogens (reference and clinical Streptococcus mutans, Actinomyces naeslundii, and Prevotella intermedia strains). Oxalic, succinic, and quinic acids, and adenine, inosine, and uridine were identified by HPLC-DAD-ESI-MS/MS. Their anti-biofilm production and preformed biofilm disaggregation activities were studied using commercial standard compounds at different concentrations. As regards S. mutans, the highest activity was shown by adenine at 5 mg mL(-1) both in the biofilm inhibition (BI 50%) and biofilm disaggregation tests (BD 20%). Considering A. naeslundii, BI values close to 80% were registered for oxalic acid at 1 mg mL(-1) and 2 mg mL(-1) and BD 50% for quinic acid at 3 mg mL(-1). A weaker activity was found against P. intermedia. Furthermore, different mixtures of the commercial standards were tested showing that the activity of a compound can be strongly and sometimes negatively affected by the presence of the other compounds.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Odontologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dentistry (hsv//eng)

Keyword

edible mushroom

organic-acids

antibacterial activity

antimicrobial

activity

antioxidant activities

liquid-chromatography

quantitative-analyses

cultivated mushrooms

pathogenic bacteria

antitumor-activity

Biochemistry & Molecular Biology

Food Science & Technology

Publication and Content Type

ref (subject category)

art (subject category)