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Calcium: A Crucial ...
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Eich, Torsten,1972-Uppsala universitet,Klinisk immunologi,Immunologi
(author)
Calcium: A Crucial Potentiator for Efficient Enzyme Digestion of the Human Pancreas
- Article/chapterEnglish2018
Publisher, publication year, extent ...
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2018-06-26
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SAGE Publications,2018
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/269658
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https://gup.ub.gu.se/publication/269658URI
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https://doi.org/10.1177/0963689718779350DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-364508URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:138824152URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-364778URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Background: Effective digestive enzymes are crucial for successful islet isolation. Supplemental proteases are essential because they synergize with collagenase for effective pancreatic digestion. The activity of these enzymes is critically dependent on the presence of Ca2+ ions at a concentration of 5-10 mM. The present study aimed to determine the Ca2+ concentration during human islet isolation and to ascertain whether the addition of supplementary Ca2+ is required to maintain an optimal Ca2+ concentration during the various phases of the islet isolation process. Methods: Human islets were isolated according to standard methods and isolation parameters. Islet quality control and the number of isolations fulfilling standard transplantation criteria were evaluated. Ca2+ was determined by using standard clinical chemistry routines. Islet isolation was performed with or without addition of supplementary Ca2+ to reach a Ca2+ of 5 mM. Results: Ca2+ concentration was markedly reduced in bicarbonate-based buffers, especially if additional bicarbonate was used to adjust the pH as recommended by the Clinical Islet Transplantation Consortium. A major reduction in Ca2+ concentration was also observed during pancreatic enzyme perfusion, digestion, and harvest. Additional Ca2+ supplementation of media used for dissolving the enzymes and during digestion, perfusion, and harvest was necessary in order to obtain the concentration recommended for optimal enzyme activity and efficient liberation of a large number of islets from the human pancreas. Conclusions: Ca2+ is to a large extent consumed during clinical islet isolation, and in the absence of supplementation, the concentration fell below that recommended for optimal enzyme activity. Ca2+ supplementation of the media used during human pancreas digestion is necessary to maintain the concentration recommended for optimal enzyme activity. Addition of Ca2+ to the enzyme blend has been implemented in the standard isolation protocols in the Nordic Network for Clinical Islet Transplantation.
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Ståhle, Magnus U.Uppsala universitet,Klinisk immunologi,Immunologi(Swepub:uu)magst207
(author)
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Gustafsson, B.Sahlgrens Univ Hosp, Dept Transplantat, Gothenburg, Sweden,Sahlgrenska, transplantation
(author)
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Horneland, R.Oslo Univ Hosp, Rikshosp, Dept Transplantat, Oslo, Norway,Rikshospitalet Oslo
(author)
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Lempinen, M.Helsinki Univ Hosp, Dept Transplantat & Liver Surg, Helsinki, Finland,Helsingfors, transplantation
(author)
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Lundgren, T.Karolinska Institutet,Karolinska, CLINTEC
(author)
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Rafael, E.Skåne Univ Hosp, Dept Surg, Transplantat Unit, Malmö, Sweden,Skånes universitetssjukhus, transplantation
(author)
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Tufveson, GunnarUppsala universitet,Transplantationskirurgi,Uppsala universitet, transplantationskirurgen(Swepub:uu)gutuf839
(author)
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von Zur-Mühlen, BengtUppsala universitet,Transplantationskirurgi,Uppsala univseristet, transplantationskirurgen(Swepub:uu)bengmuhl
(author)
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Olerud, JohanUppsala universitet,Klinisk immunologi,Uppsala universitet, immunologen(Swepub:uu)joole425
(author)
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Scholz, H.Oslo Univ Hosp, Rikshosp, Dept Transplantat, Oslo, Norway,Rikshopsitalet Oslo, transplantation
(author)
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Korsgren, OlleUppsala universitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Klinisk immunologi,Univ Gothenburg, Dept Biomed, Gothenburg, Sweden,Uppsala universitet, immunologen(Swepub:uu)ollekors
(author)
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Uppsala universitetKlinisk immunologi
(creator_code:org_t)
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In:Cell Transplantation: SAGE Publications27:7, s. 1031-10380963-68971555-3892
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Rafael, E.
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