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Interaction of B ce...
Interaction of B cells with intracellular pathogen Francisella tularensis.
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Krocova, Zuzana (author)
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- Härtlova, Anetta (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Souckova, Dagmar (author)
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Zivna, Lucie (author)
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Kroca, Michal (author)
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Rudolf, Emil (author)
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Macela, Ales (author)
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Stulik, Jiri (author)
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Microbial pathogenesis. - : Elsevier BV. - 0882-4010. ; 45:2, s. 79-85
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https://doi.org/10.1...
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Abstract
Subject headings
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- Immunity to Francisella tularensis is largely mediated by T lymphocytes but an important role of B lymphocytes in early stage of infection was previously uncovered. We wanted to find out if F. tularensis is able to infect B cells and/or influence them by direct contact. To investigate this possibility we infected B cell lines from mouse (A20) or humans (Ramos RA-1), or primary mouse spleen cells, with F. tularensis LVS and F. tularensis FSC200 in vitro. In all cases, we detected bacteria on the cell surface and inside the B cells using transmission electron microscopy. More than 20% cells were infected by microbes after 24h. The number of bacteria, determined by CFU, increased about 1 log during 24h. Infection with live bacteria led to apoptosis of Ramos cells and mouse CD19(+) spleen cells. Approximately 30% of cells were apoptotic after 24h and 70% after 48 h, independently of the F. tularensis strain, while only 10% of non-infected cell were apoptotic at either time point. Apoptosis was confirmed by Western blot using anti-PARP antibodies. Thus, this study demonstrates unique phenomenon - namely, the ability of the intracellular pathogen F. tularensis to invade and induce apoptosis in B cells.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- Animals
- Apoptosis
- B-Lymphocytes
- immunology
- microbiology
- physiology
- Cell Line
- Francisella tularensis
- immunology
- physiology
- Humans
- Mice
- Microscopy
- Electron
- Transmission
Publication and Content Type
- ref (subject category)
- art (subject category)
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