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Human Co- and Tripl...
Human Co- and Triple-Culture Model of the Alveolar-Capillary Barrier on a Basement Membrane Mimic
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Dohle, E. (author)
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Singh, S. (author)
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Nishigushi, A. (author)
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Fischer, T. (author)
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Wessling, M. (author)
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Moller, M. (author)
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Sader, R. (author)
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Kasper, J. (author)
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Ghanaati, S. (author)
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- Kirkpatrick, Charles James (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap,Institute of Clinical Sciences, Department of Biomaterials
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2018
- 2018
- English.
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In: Tissue Engineering Part C-Methods. - : Mary Ann Liebert Inc. - 1937-3384 .- 1937-3392. ; 24:9, s. 495-503
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https://doi.org/10.1...
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Abstract
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- The development of an in vitro model resembling the alveolar-capillary barrier might be a highly beneficial tool to study lung physiology as well as the immune response of the lung to infection or after exposure to nanoparticles. This study is based on an in vitro alveolar barrier developed on a basement membrane mimic, composed of ultrathin nanofiber meshes generated via electrospinning using bioresorbable poly(epsilon-caprolactone). As cellular components, NCI H441, resembling the alveolar epithelial cells, and ISO-HAS-1, an endothelial cell line, were used to perform bipolar coculture experiments for a total cultivation period of 14 days. In addition to immunohistochemical and immunofluorescent studies, transepithelial electrical resistance (TER) and transport capabilities of the in vitro model system were investigated. Alveolar barrier function could be clearly determined for the postulated bipolar coculture system on the basement membrane mimic, since TER increased during the course of bipolar cultivation. Furthermore, to gain first insights into possible lung inflammatory reactions in vitro, this coculture model was further expanded by a human leukemia monocyte cell line (THP-1). This triple-culture system was able to maintain adequately the barrier properties of the bipolar coculture, thus making this in vitro model consisting of epithelial, endothelial, and immune cells on a basement membrane mimic a promising basis for further studies in tissue engineering.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- basement membrane mimic
- alveolar-capillary barrier
- in vitro co-culture model
- in-vitro
- pneumococcal pneumonia
- epithelial barrier
- endothelial-cells
- macrophage
- scaffolds
- adhesion
- lines
- Cell Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Dohle, E.
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Singh, S.
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Nishigushi, A.
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Fischer, T.
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Wessling, M.
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Moller, M.
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show more...
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Sader, R.
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Kasper, J.
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Ghanaati, S.
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Kirkpatrick, Cha ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomaterials Sci ...
- Articles in the publication
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Tissue Engineeri ...
- By the university
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University of Gothenburg