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Electrophysiologica...
Electrophysiological properties of human beta-cell lines EndoC-beta H1 and -beta H2 conform with human beta-cells
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Hastoy, B. (author)
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Godazgar, M. (author)
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Clark, A. (author)
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Nylander, V. (author)
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Spiliotis, I. (author)
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van de Bunt, M. (author)
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Chibalina, M. V. (author)
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Barrett, A. (author)
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Burrows, C. (author)
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Tarasov, A. I. (author)
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Scharfmann, R. (author)
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Gloyn, A. L. (author)
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- Rorsman, Patrik, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology
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(creator_code:org_t)
- 2018-11-19
- 2018
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 8
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https://www.nature.c...
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Abstract
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- Limited access to human islets has prompted the development of human beta cell models. The human beta cell lines EndoC-beta H1 and EndoC-beta H2 are increasingly used by the research community. However, little is known of their electrophysiological and secretory properties. Here, we monitored parameters that constitute the glucose-triggering pathway of insulin release. Both cell lines respond to glucose (6 and 20 mM) with 2- to 3-fold stimulation of insulin secretion which correlated with an elevation of [Ca2+](i), membrane depolarisation and increased action potential firing. Similar to human primary beta cells, K-ATP channel activity is low at 1mM glucose and is further reduced upon increasing glucose concentration; an effect that was mimicked by the K-ATP channel blocker tolbutamide. The upstroke of the action potentials reflects the activation of Ca2+ channels with some small contribution of TTX-sensitive Na+ channels. The repolarisation involves activation of voltage-gated Kv2.2 channels and large-conductance Ca2+-activated K+ channels. Exocytosis presented a similar kinetics to human primary beta cells. The ultrastructure of these cells shows insulin vesicles composed of an electrondense core surrounded by a thin clear halo. We conclude that the EndoC-beta H1 and -beta H2 cells share many features of primary human beta-cells and thus represent a useful experimental model.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- insulin-secretion
- glucose
- responses
- Science & Technology - Other Topics
- an pm
- 1973
- diabetologia
- v9
- p115
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Hastoy, B.
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Godazgar, M.
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Clark, A.
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Nylander, V.
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Spiliotis, I.
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van de Bunt, M.
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show more...
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Chibalina, M. V.
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Barrett, A.
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Burrows, C.
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Tarasov, A. I.
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Scharfmann, R.
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Gloyn, A. L.
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Rorsman, Patrik, ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Physiology
- Articles in the publication
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Scientific Repor ...
- By the university
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University of Gothenburg