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Antibody-based meth...
Antibody-based methods for the measurement of α-synuclein concentration in human cerebrospinal fluid - method comparison and round robin study.
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Mollenhauer, Brit (author)
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Bowman, Frederick DuBois (author)
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Drake, Daniel (author)
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Duong, Jimmy (author)
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- Blennow, Kaj, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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El-Agnaf, Omar (author)
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Shaw, Leslie M (author)
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Masucci, Jennifer (author)
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Taylor, Peggy (author)
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Umek, Robert M (author)
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Dunty, Jill M (author)
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Smith, Chris L (author)
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Stoops, Erik (author)
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Vanderstichele, Hugo (author)
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Schmid, Adrian W (author)
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Moniatte, Marc (author)
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Zhang, Jing (author)
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Kruse, Niels (author)
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Lashuel, Hilal A (author)
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Teunissen, Charlotte (author)
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Schubert, Tanja (author)
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Dave, Kuldip D (author)
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Hutten, Samantha J (author)
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- Zetterberg, Henrik, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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(creator_code:org_t)
- 2018-11-13
- 2019
- English.
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In: Journal of neurochemistry. - : Wiley. - 1471-4159 .- 0022-3042. ; 149:1, s. 126-138
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Abstract
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- α-Synuclein is the major component of Lewy bodies and a candidate biomarker for neurodegenerative diseases in which Lewy bodies are common, including Parkinson's disease and dementia with Lewy bodies. A large body of literature suggests that these disorders are characterized by reduced concentrations of α-synuclein in cerebrospinal fluid (CSF), with overlapping concentrations compared to healthy controls and variability across studies. Several reasons can account for this variability, including technical ones, such as inter-assay and inter-laboratory variation (reproducibility). We compared four immunochemical methods for the quantification of α-synuclein concentration in 50 unique CSF samples. All methods were designed to capture most of the existing α-synuclein forms in CSF ('total' α-synuclein). Each of the four methods showed high analytical precision, excellent correlation between laboratories (R2 0.83-0.99), and good correlation with each other (R2 0.64-0.93), although the slopes of the regression lines were different between the four immunoassays. The use of common reference CSF samples decreased the differences in α-synuclein concentration between detection methods and technologies. Pilot data on an immunoprecipitation mass spectrometry (IP-MS) method isalso presented. Our results suggest that the four immunochemical methods and the IP-MS method measure similarforms of α-synuclein and that a common reference materialwould allow harmonization of results between immunoassays.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Mollenhauer, Bri ...
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Bowman, Frederic ...
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Drake, Daniel
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Duong, Jimmy
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Blennow, Kaj, 19 ...
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El-Agnaf, Omar
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show more...
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Shaw, Leslie M
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Masucci, Jennife ...
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Taylor, Peggy
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Umek, Robert M
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Dunty, Jill M
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Smith, Chris L
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Stoops, Erik
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Vanderstichele, ...
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Schmid, Adrian W
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Moniatte, Marc
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Zhang, Jing
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Kruse, Niels
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Lashuel, Hilal A
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Teunissen, Charl ...
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Schubert, Tanja
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Dave, Kuldip D
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Hutten, Samantha ...
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Zetterberg, Henr ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Journal of neuro ...
- By the university
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University of Gothenburg