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Biology/Disease-Dri...
Biology/Disease-Driven Initiative on Protein-Aggregation Diseases of the Human Proteome Project: Goals and Progress to Date
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- Boersema, P. J. (author)
- ETH Zürich
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- Melnik, A. (author)
- ETH Zürich
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- Hazenberg, B. P. C. (author)
- University Medical Center Groningen
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- Rezeli, Melinda (author)
- Lund University,Lunds universitet,Clinical Protein Science and Imaging,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Marko-Varga, György (author)
- Lund University,Lunds universitet,Clinical Protein Science and Imaging,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Kamiie, J. (author)
- Azabu University
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- Portelius, Erik, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,Sahlgrenska University Hospital,Sahlgrenska Academy
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- Blennow, Kaj, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,Sahlgrenska University Hospital,Sahlgrenska Academy
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- Zubarev, R. A. (author)
- Karolinska Institutet
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- Polymenidou, M. (author)
- University of Zurich
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Picotti, P. (author)
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(creator_code:org_t)
- 2018-08-23
- 2018
- English.
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In: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 17:12, s. 4072-4084
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Abstract
Subject headings
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- The Biology/Disease-driven (B/D) working groups of the Human Proteome Project are alliances of research groups aimed at developing or improving proteomic tools to support specific biological or disease-related research areas. Here, we describe the activities and progress to date of the B/D working group focused on protein aggregation diseases (PADs). PADs are characterized by the intra- or extracellular accumulation of aggregated proteins and include devastating diseases such as Parkinson's and Alzheimer's disease and systemic amyloidosis. The PAD B/D working group aims for the development of proteomic assays for the quantification of aggregation-prone proteins involved in PADs to support basic and clinical research on PADs. Because the proteins in PADs undergo aberrant conformational changes, a goal is to quantitatively resolve altered protein structures and aggregation states in complex biological specimens. We have developed protein-extraction protocols and a set of mass spectrometric (MS) methods that enable the detection and quantification of proteins involved in the systemic and localized amyloidosis and the probing of aberrant protein conformational transitions in cell and tissue extracts. In several studies, we have demonstrated the potential of MS-based proteomics approaches for specific and sensitive clinical diagnoses and for the subtyping of PADs. The developed methods have been detailed in both protocol papers and manuscripts describing applications to facilitate implementation by nonspecialized laboratories, and assay coordinates are shared through public repositories and databases. Clinicians actively involved in the PAD working group support the transfer to clinical practice of the developed methods, such as assays to quantify specific disease related proteins and their fragments in biofluids and multiplexed MS-based methods for the diagnosis and typing of systemic amyloidosis. We believe that the increasing availability of tools to precisely measure proteins involved in PADs will positively impact research on the molecular bases of these diseases and support early disease diagnosis and a more-confident subtyping.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- protein-aggregation diseases
- Human Proteome Project
- Biology/Disease-driven HPP
- Alzheimer's
- amyloid precursor protein
- cerebrospinal-fluid biomarker
- gamma-secretase modulator
- alzheimers-disease
- apolipoprotein-e
- mass-spectrometry
- structural-changes
- human brain
- b/d-hpp
- a-beta
- Alzheimer's disease
- amyotrophic lateral sclerosis
- frontotemporal dementia
- Parkinson's disease
- systemic amyloidosis
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Boersema, P. J.
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Melnik, A.
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Hazenberg, B. P. ...
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Rezeli, Melinda
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Marko-Varga, Gyö ...
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Kamiie, J.
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show more...
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Portelius, Erik, ...
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Blennow, Kaj, 19 ...
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Zubarev, R. A.
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Polymenidou, M.
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Picotti, P.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Journal of Prote ...
- By the university
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University of Gothenburg
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Karolinska Institutet
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Lund University