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Successful optimization of reconstruction parameters in structured illumination microscopy – A practical guide
- Karras, C. (author)
-
- Smedh, Maria, 1968 (author)
- Gothenburg University,Göteborgs universitet,Core Facilities, Centre for Cellular Imaging,Core Facilities, Centre for Cellular Imaging
- Förster, R. (author)
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- Heintzmann, R. (author)
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- (creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English.
- In: Optics Communications. - : Elsevier BV. - 0030-4018. ; 436, s. 69-75
- Journal article (peer-reviewed)
Abstract
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- The impact of the different reconstruction parameters in super-resolution structured illumination microscopy (SIM) on image artifacts is carefully analyzed. These parameters comprise the Wiener filter parameter, an apodization function, zero-frequency suppression and modifications of the optical transfer function. A detailed investigation of the reconstructed image spectrum is concluded to be suitable for identifying artifacts. For this purpose, two samples, an artificial test slide and a more realistic biological system, were used to characterize the artifact classes and their correlation with the image spectra as well as the reconstruction parameters. In addition, a guideline for efficient parameter optimization is suggested and the implementation of the parameters in selected up-to-date processing packages (proprietary and open-source) is depicted. © 2018 The Authors
Subject headings
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- Fluorescence microscopy
- Image reconstruction
- Parameter estimation
- Structured illumination microscopy
- Super resolution
- Optical resolving power
- Optical transfer function
- Apodization function
- Image artifacts
- Parameter optimization
- Reconstructed image
- Reconstruction parameters
- Structured illumination microscopies (SIM)
Publication and Content Type
- ref (subject category)
- art (subject category)
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