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Novel ELISA for thr...
Novel ELISA for thrombospondin type 1 domain-containing 7A autoantibodies in membranous nephropathy
- Article/chapterEnglish2019
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LIBRIS-ID:oai:gup.ub.gu.se/278425
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https://gup.ub.gu.se/publication/278425URI
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https://doi.org/10.1016/j.kint.2018.10.024DOI
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Subject category:art swepub-publicationtype
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Autoantibodies against phospholipase A2 receptor 1 (PLA2R1) and thrombospondin type 1 domain-containing 7A (THSD7A) are emerging as biomarkers to classify membranous nephropathy (MN) and to predict outcome or response to treatment. Anti-THSD7A autoantibodies are detected by Western blot and indirect immunofluorescence test (IIFT). Here, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) optimized for quantitative detection of anti-THSD7A autoantibodies. Among 1012 biopsy-proven MN patients from 6 cohorts, 28 THSD7A-positive patients were identified by ELISA, indicating a prevalence of 2.8%. By screening additional patients, mostly referred because of PLA2R1-unrelated MN, we identified 21 more cases, establishing a cohort of 49 THSD7A-positive patients. Twenty-eight patients (57%) were male, and male patients were older than female patients (67 versus 49 years). Eight patients had a history of malignancy, but only 3 were diagnosed with malignancy within 2 years of MN diagnosis. We compared the results of ELISA, IIFT, Western blot, and biopsy staining, and found a significant correlation between ELISA and IIFT titers. Anti-THSD7A autoantibodies were predominantly IgG4 in all patients. Eight patients were double positive for THSD7A and PLA2R1. Levels of anti-THSD7A autoantibodies correlated with disease activity and with response to treatment. Patients with high titer at baseline had poor clinical outcome. In a subgroup of patients with serial titers, persistently elevated anti-THSD7A autoantibodies were observed in patients who did not respond to treatment or did not achieve remission. We conclude that the novel anti-THSD7A ELISA can be used to identify patients with THSD7A-associated MN and to monitor autoantibody titers during treatment.
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Seitz-Polski, B.
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Justino, J.
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Dolla, G.
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Payre, C.
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Jourde-Chiche, N.
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Van de Logt, A. E.
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Booth, C.
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Rigby, E.
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Lönnbro-Widgren, JennieGothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine(Swepub:gu)xlonnj
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Nyström, Jenny,1972Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine(Swepub:gu)xnysje
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Mariat, C.
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Cui, Z.
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Wetzels, J. F. M.
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Ghiggeri, G.
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Beck, L. H.
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Ronco, P.
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Debiec, H.
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Lambeau, G.
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Göteborgs universitetInstitutionen för medicin, avdelningen för molekylär och klinisk medicin
(creator_code:org_t)
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In:Kidney International: Elsevier BV95:3, s. 666-6790085-2538
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Zaghrini, C.
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Seitz-Polski, B.
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Justino, J.
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Dolla, G.
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Payre, C.
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Jourde-Chiche, N ...
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Van de Logt, A. ...
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Booth, C.
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Rigby, E.
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Lönnbro-Widgren, ...
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Nyström, Jenny, ...
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Mariat, C.
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Cui, Z.
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Wetzels, J. F. M ...
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Ghiggeri, G.
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Beck, L. H.
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Ronco, P.
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Debiec, H.
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Lambeau, G.
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- MEDICAL AND HEALTH SCIENCES
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and Clinical Medicin ...
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Kidney Internati ...
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University of Gothenburg