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HOTMAQ: A Multiplex...
HOTMAQ: A Multiplexed Absolute Quantification Method for Targeted Proteomics
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Zhong, X. F. (author)
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Yu, Q. Y. (author)
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Ma, F. F. (author)
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Frost, D. C. (author)
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Lu, L. (author)
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Chen, Z. W. (author)
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- Zetterberg, Henrik, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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Carlsson, C. (author)
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Okonkwo, O. (author)
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Li, L. J. (author)
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(creator_code:org_t)
- 2019-01-04
- 2019
- English.
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In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 91:3, s. 2112-2119
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Abstract
Subject headings
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- Absolute quantification in targeted proteomics is challenging due to a variety of factors, including low specificity in complex backgrounds, limited analytical throughput, and wide dynamic range. To address these problems, we developed a hybrid offset-triggered multiplex absolute quantification (HOTMAQ) strategy that combines cost-effective mass difference and isobaric tags to enable simultaneous construction of an internal standard curve in the MS1 precursor scan, real-time identification of peptides at the MS2 level, and mass offset-triggered accurate quantification of target proteins in synchronous precursor selection (SPS)-MS3 spectra. This approach increases the analytical throughput of targeted quantitative proteomics by up to 12-fold. The HOTMAQ strategy was employed to verify candidate protein biomarkers in preclinical Alzheimer's disease with high accuracy. The greatly enhanced throughput and quantitative performance, paired with sample flexibility, makes HOTMAQ broadly applicable to targeted peptidomics, proteomics, and phosphoproteomics.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Keyword
- cerebrospinal-fluid biomarkers
- alzheimer-disease risk
- apolipoprotein-e
- mass-spectrometry
- proteins
- peptides
- plasma
- csf
- phosphoproteins
- discovery
- Chemistry
- lstein mf
- 1975
- journal of psychiatric research
- v12
- p189
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Zhong, X. F.
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Yu, Q. Y.
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Ma, F. F.
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Frost, D. C.
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Lu, L.
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Chen, Z. W.
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show more...
-
Zetterberg, Henr ...
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Carlsson, C.
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Okonkwo, O.
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Li, L. J.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Neurology
- Articles in the publication
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Analytical Chemi ...
- By the university
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University of Gothenburg