C-band-positive double minutes have kinetochore proteins.

• Double minutes (DMs) have been shown generally to lack centromeres. In the present paper we report on C-band-positive DMs in Colcemid-resistant SEWA cells. These DMs were heavily labeled after in situ hybridization with mouse major satellite DNA and, furthermore, reacted positively with antikinetochore antibodies. Upon antikinetochore antibody labeling, the immunofluorescent signals of some DMs were strong, indicating that they had functional centromeres.

Subject headings

NATURVETENSKAP  -- Biologi -- Genetik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Genetics (hsv//eng)

Keyword

Animals

Autoantibodies

blood

CREST Syndrome

blood

immunology

Centromere

genetics

Chromosome Banding

Clone Cells

DNA

Satellite

Demecolcine

toxicity

Drug Resistance

Multiple

genetics

Humans

Kinetochores

physiology

Metaphase

Mice

Publication and Content Type

ref (subject category)

art (subject category)