Adherence of RFD-1 positive dendritic cells to the basal surface of thyroid follicular cells in Graves' disease.

Mölne, Johan,1958Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin , Avdelningen för patologi,Institute of Laboratory Medicine, Dept of Pathology (author)

HLA-DR positive cells infiltrating Graves' thyroid tissue were examined for their expression of cell-specific immunological markers using light and electron microscopic immunostaining of frozen sections and isolated open thyroid follicles. Graves' glands (n = 21) were enriched of CD68 and Leu-M5/CD11c positive monocytes/macrophages as well as CD4 and CD8 lymphocytes. These cell types were distributed juxta-follicular as well as in other tissue areas. Only the RFD-1 antibody, considered to label antigen-presenting cells including dendritic cells, identified cells invariably located close to the interstitial follicular surface. After follicle isolation, RFD-1 cells were enriched compared to Leu-M5 cells and exclusively adherent to the follicular epithelium. The plasma membrane of RFD-1 positive cells were in intimate contact with the basolateral membrane of the thyrocytes, sometimes extending deeply into the intercellular space of the epithelium. Parallel labelling experiments suggested that the follicle-adhering RFD-1 cells also expressed HLA-DR. Our findings show that in human thyroid glands with Graves' disease RFD-1 positive cells with a dendritic morphology establish direct contact with the follicular epithelium. In view of the fact that both HLA-DR and RFD-1 are associated with antigen-presenting functions it is suggested that a direct interaction of dendritic cells with thyrocytes might be an important component of the autoimmune reaction in Graves' disease.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Antibodies
Monoclonal
immunology
Antigen-Presenting Cells
immunology
pathology
Autoimmune Diseases
immunology
pathology
Biomarkers
Cell Adhesion
Cell Differentiation
Cell Polarity
Dendritic Cells
pathology
Epithelium
immunology
pathology
Graves Disease
immunology
pathology
HLA-DR Antigens
analysis
Humans
Lymphocyte Subsets
immunology
pathology
Macrophages
immunology
pathology
Thyroid Gland
immunology
pathology

Jansson, S (author)
Ericson, L E (author)
Nilsson, M (author)
Göteborgs universitetInstitutionen för laboratoriemedicin , Avdelningen för patologi (creator_code:org_t)

By the author/editor: Mölne, Johan, 19 ...; Jansson, S; Ericson, L E; Nilsson, M

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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