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E-cadherin: a diffe...
E-cadherin: a differentiation marker in thyroid malignancies.
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Brabant, G (author)
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Hoang-Vu, C (author)
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Cetin, Y (author)
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Dralle, H (author)
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Scheumann, G (author)
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- Mölne, Johan, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin , Avdelningen för patologi,Institute of Laboratory Medicine, Dept of Pathology
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Hansson, G (author)
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Jansson, S (author)
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Ericson, L E (author)
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Nilsson, M (author)
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(creator_code:org_t)
- 1993
- 1993
- English.
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In: Cancer research. - 0008-5472. ; 53:20, s. 4987-93
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https://gup.ub.gu.se...
Abstract
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- Loss of E-cadherin (uvomorulin), a Ca(2+)-dependent cell adhesion molecule required for normal epithelial function, has been attributed a pathogenetic role in tumor invasion. The expression of E-cadherin was studied in normal and neoplastic follicular epithelium of the human thyroid by Northern blot analysis and immunofluorescence on frozen tissue sections. In the normal thyroid (n = 10) and in benign thyroid disorders (n = 21; toxic diffuse goitre; multinodular goitre; follicular adenomas), E-cadherin mRNA levels were equally high and the follicles were generally stained, mainly along the lateral surface of the epithelial cells, by the anti-E-cadherin monoclonal antibody. In anaplastic thyroid carcinomas (n = 6) E-cadherin expression was very low or lacking. In papillary carcinomas (n = 23), E-cadherin mRNA levels varied from nearly normal to highly reduced, which roughly correlated with the overall immunofluorescence intensity. However, the immunostaining also revealed a heterogeneous "all-or-nothing" expression of E-cadherin among adjacent cells in the same tumor. In the follicular carcinomas (n = 9), E-cadherin mRNA levels were in general rather high but the immunostaining varied considerably. A few papillary and follicular tumors lacked immunoreactive E-cadherin in spite of high mRNA levels. In oxyphilic (Hürthle) cell tumors, comprising both adenomas (n = 4) and carcinomas (n = 2), E-cadherin immunoreactivity was reduced and distributed intracellularly rather than at the cell surface. The expression of E-cadherin in relapsing thyroid carcinomas and in tumors with metastatic spreading was, irrespective of the histiotype, low or lacking. Sequential Northern analysis revealed a close correlation between the expression levels of E-cadherin and the thyrotropin receptor. Together, the data suggest that in human thyroid malignancies both gene expression and posttranscriptional control of E-cadherin may be impaired.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- Adenoma
- metabolism
- pathology
- Biomarkers
- Tumor
- analysis
- Blotting
- Northern
- Cadherins
- analysis
- biosynthesis
- Carcinoma
- Papillary
- metabolism
- pathology
- Cell Differentiation
- Fluorescent Antibody Technique
- Humans
- RNA
- Messenger
- analysis
- metabolism
- Receptors
- Thyrotropin
- biosynthesis
- Thyroid Gland
- cytology
- metabolism
- Thyroid Neoplasms
- metabolism
- pathology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Brabant, G
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Hoang-Vu, C
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Cetin, Y
-
Dralle, H
-
Scheumann, G
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Mölne, Johan, 19 ...
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show more...
-
Hansson, G
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Jansson, S
-
Ericson, L E
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Nilsson, M
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
- Articles in the publication
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Cancer research
- By the university
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University of Gothenburg