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Coherent diffractive imaging of microtubules using an X-ray laser.
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- Brändén, Gisela, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Hammarin, Greger, 1981 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Harimoorthy, Rajiv (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Malmerberg, Erik (author)
- Barty, Anton (author)
- Tångefjord, Stefan (author)
- DePonte, Daniel P (author)
- Seuring, Carolin (author)
- White, Thomas A (author)
- Stellato, Francesco (author)
- Bean, Richard (author)
- Beyerlein, Kenneth R (author)
- Chavas, Leonard M G (author)
- Fleckenstein, Holger (author)
- Gati, Cornelius (author)
- Ghoshdastider, Umesh (author)
- Gumprecht, Lars (author)
- Oberthür, Dominik (author)
- Popp, David (author)
- Seibert, Marvin (author)
- Tilp, Thomas (author)
- Messerschmidt, Marc (author)
- Williams, Garth J (author)
- Loh, N Duane (author)
- Chapman, Henry N (author)
- Zwart, Peter (author)
- Liang, Mengning (author)
- Boutet, Sébastien (author)
- Robinson, Robert C (author)
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- (creator_code:org_t)
- 2019-06-13
- 2019
- English.
- In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 10:1
- Journal article (peer-reviewed)
Abstract
Subject headings
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- X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Tilp, Thomas
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