Mitochondrial protein enriched extracellular vesicles discovered in human melanoma tissues can be detected in patient plasma

Jang, Su Chul,1984Gothenburg University,Göteborgs universitet,Krefting Research Centre (author)

Extracellular vesicles (EVs), including exosomes and microvesicles, are secreted from all cells, and convey messages between cells in health and disease. However, the diversity of EV subpopulations is only beginning to be explored. Since EVs have been implicated in tumour microenvironmental communication, we started to determine the diversity of EVs specifically in this tissue. To do this, we isolated EVs directly from patient melanoma metastatic tissues. Using EV membrane isolation and mass spectrometry analysis, we discovered enrichment of mitochondrial membrane proteins in the melanoma tissue-derived EVs, compared to non-melanoma-derived EVs. Interestingly, two mitochondrial inner membrane proteins MT-CO2 (encoded by the mitochondrial genome) and COX6c (encoded by the nuclear genome) were highly prevalent in the plasma of melanoma patients, as well as in ovarian and breast cancer patients. Furthermore, this subpopulation of EVs contains active mitochondrial enzymes. In summary, tumour tissues are enriched in EVs with mitochondrial membrane proteins and these mitochondrial membrane proteins can be detected in plasma and are increased in melanoma, ovarian cancer as well as breast cancer.

Crescitelli, Rossella,1985Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xcrero (author)
Cvjetkovic, AleksanderGothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xcvjal (author)
Belgrano, ValerioGothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery(Swepub:gu)xbelgv (author)
Olofsson Bagge, Roger,1978Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery(Swepub:gu)xoloro (author)
Sundfeldt, Karin,1962Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Sahlgrenska Cancer Center,Institute of Clinical Sciences, Department of Obstetrics and Gynecology(Swepub:gu)xsukar (author)
Ochiya, T. (author)
Kalluri, R. (author)
Lötvall, Jan,1956Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xlotja (author)
Göteborgs universitetKrefting Research Centre (creator_code:org_t)

By the author/editor: Jang, Su Chul, 1 ...; Crescitelli, Ros ...; Cvjetkovic, Alek ...; Belgrano, Valeri ...; Olofsson Bagge, ...; Sundfeldt, Karin ...; show more...; Ochiya, T.; Kalluri, R.; Lötvall, Jan, 19 ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

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