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Pre-analytical stability of novel cerebrospinal fluid biomarkers

Willemse, E. A. J. (author)
Vermeiren, Y. (author)
Garcia-Ayllon, M. S. (author)
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Bridel, C. (author)
De Deyn, P. P. (author)
Engelborghs, S. (author)
van der Flier, W. M. (author)
Jansen, E. E. W. (author)
Lopez-Font, I. B. (author)
Mendes, V. (author)
Manadas, B. (author)
de Roeck, N. (author)
Saez-Valero, J. (author)
Struys, E. A. (author)
Vanmechelen, E. (author)
Andreasson, Ulf, 1968 (author)
Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
Teunissen, C. E. (author)
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 (creator_code:org_t)
Elsevier BV, 2019
2019
English.
In: Clinica Chimica Acta. - : Elsevier BV. - 0009-8981. ; 497, s. 204-211
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  • Journal article (peer-reviewed)
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  • Stability of the cerebrospinal fluid (CSF) composition under different pre-analytical conditions is relevant for the diagnostic potential of biomarkers. Our aim was to examine the pre-analytical stability of promising CSF biomarkers that are currently evaluated for their discriminative use in various neurological diseases. Pooled CSF was aliquoted and experimentally exposed to delayed storage: 0, 1, 2, 4, 24, 72, or 168 h at 4 °C or room temperature (RT), or 1–4 months at −20 °C; or up to 7 freeze/thaw (f/t) cycles, before final storage at −80 °C. Eleven CSF biomarkers were screened using immunoassays, liquid chromatography, or enzymatic methods. Levels of neurogranin (truncP75), chitinase-3-like protein (YKL-40), beta-site amyloid precursor protein cleaving enzyme 1 (BACE1), acetylcholinesterase (AChE) enzymatic activity, theobromine, secreted protein acidic and rich in cysteine-like 1 (SPARCL-1) and homovanillic acid (HVA) levels were not affected by the applied storage conditions. 3-Methoxy-4-hydroxyphenylglycol (MHPG) levels linearly and strongly decreased after 4 h at RT (−10%) or 24 h at 4 °C (−27%), and with 6% after every f/t cycle. 5-Methyltetrahydrofolate (5-MTHF) (−29% after 1 week at RT) and 5-hydroxyindoleacetic acid levels (5-HIAA) (−16% after 1 week at RT) were reduced and 3,4-dihydroxyphenylacetic acid (DOPAC) levels (+22% after 1 week at RT) increased, but only after >24 h at RT. Ten out of eleven potential CSF novel biomarkers showed very limited change under common storage and f/t conditions, suggesting that these CSF biomarkers can be trustfully tested under the pre-analytical conditions present across different cohorts. © 2019 Elsevier B.V.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Annan klinisk medicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Other Clinical Medicine (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Neurosciences (hsv//eng)

Keyword

Assay validation
Biomarkers
Human cerebrospinal fluid
Neurodegenerative diseases
Pre-analytical stability

Publication and Content Type

ref (subject category)
art (subject category)

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