Search: onr:"swepub:oai:gup.ub.gu.se/285722" >
Endosomal signallin...
Endosomal signalling via exosome surface TGF beta-1
-
- Shelke, Ganesh V, 1986 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Krefting Research Centre,Institute of Clinical Sciences, Department of Surgery,Univ Gothenburg, Sahlgrenska Acad, Krefting Res Ctr, Inst Med, Gothenburg, Sweden.;Univ Gothenburg, Sahlgrenska Acad, Inst Clin Sci, Dept Surg, Gothenburg, Sweden.
-
- Yin, Yanan (author)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre,Univ Gothenburg, Sahlgrenska Acad, Krefting Res Ctr, Inst Med, Gothenburg, Sweden.;Shanghai Jiao Tong Univ, Sch Med, Dept Biochem & Mol Cell Biol, Shanghai, Peoples R China.
-
- Jang, Su Chul, 1984 (author)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre,Univ Gothenburg, Sahlgrenska Acad, Krefting Res Ctr, Inst Med, Gothenburg, Sweden.
-
show more...
-
- Lässer, Cecilia, 1981 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Krefting Research Centre,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition,Univ Gothenburg, Sahlgrenska Acad, Krefting Res Ctr, Inst Med, Gothenburg, Sweden.
-
- Wennmalm, Stefan, 1970- (author)
- KTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab
-
- Hoffmann, H. J. (author)
- Aarhus Univ, Dept Clin Med, Aarhus, Denmark.;Aarhus Univ Hosp, Dept Resp & Allergy, Aarhus, Denmark.
-
- Li, L. (author)
- Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 1, Dept Lab Med, Shanghai, Peoples R China.
-
- Gho, Y. S. (author)
- Pohang Univ Sci & Technol, Dept Life Sci, Pohang, South Korea.
-
- Nilsson, Jonas A, 1971 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery,Univ Gothenburg, Sahlgrenska Acad, Inst Clin Sci, Dept Surg, Gothenburg, Sweden.
-
- Lötvall, Jan, 1956 (author)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre,Univ Gothenburg, Sahlgrenska Acad, Krefting Res Ctr, Inst Med, Gothenburg, Sweden.
-
show less...
-
(creator_code:org_t)
- 2019-09-20
- 2019
- English.
-
In: Journal of Extracellular Vesicles. - : Wiley. - 2001-3078. ; 8:1
- Related links:
-
https://www.tandfonl...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor beta-1 (TGF beta-1) on their surfaces. The latent form of TGF beta-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGF beta-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGF beta-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGF beta-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Mast cells
- extracellular vesicles
- exosomes
- mesenchymal stem cells
- tumour growth factor beta-1
- cellular localization
- endosomal signalling
- proteoglycan
- mesenchymal stem-cells
- heparan-sulfate proteoglycans
- growth-factor-beta
- tgf-beta
- mediated transfer
- cancer exosomes
- internalization
- fibroblast
- phenotype
- migration
- Cell Biology
- Mast cells
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Shelke, Ganesh V ...
-
Yin, Yanan
-
Jang, Su Chul, 1 ...
-
Lässer, Cecilia, ...
-
Wennmalm, Stefan ...
-
Hoffmann, H. J.
-
show more...
-
Li, L.
-
Gho, Y. S.
-
Nilsson, Jonas A ...
-
Lötvall, Jan, 19 ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Journal of Extra ...
- By the university
-
University of Gothenburg
-
Royal Institute of Technology